MAPK Signal Transduction Pathway Regulation : A Novel Mechanism of Rat HSC-T6 Cell Apoptosis Induced by FUZHENGHUAYU Tablet
المؤلفون المشاركون
Li, Yue
Cheng, Jun
Zhang, Xiaoli
Du, Hongbo
Wang, Qi
Liu, Shunai
Li, Min
Gao, Ping
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-05-16
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
FUZHENGHUAYU Tablets have been widely used in the treatment of liver fibrosis in China.
Here, we investigate the apoptotic effect of FUZHENGHUAYU Tablet in rat liver stellate cell line HSC-T6.
HSC-T6 cells were incubated with control serum or drug serum from rats fed with 0.9% NaCl or FUZHENGHUAYU Tablet, respectively.
Cells exposed to drug serum showed higher proportions of early and late apoptotic cells than controls.
The mRNA levels of collagens I and III, TGF-β1 and α-SMA were reduced by drug serum compared to control serum.
Differentially expressed mRNAs and miRNAs were analyzed by microarray and sequencing, respectively.
We identified 334 differentially expressed mRNAs and also 60 GOs and two pathways related to the mRNAs.
Seventy-five differentially expressed miRNAs were down-regulated by drug serum and 1963 target genes were predicted.
134 GOs up-regulated in drug serum group were linked to miRNA targets, and drug serum also regulated 43 miRNA signal transduction pathways.
Protein levels were evaluated by Western blot.
Drug serum down-regulated (phospho-SAPK/JNK)/(SAPK/JNK) and up-regulated phospho-p38/p38 ratios.
The study showed that FUZHENGHUAYU Tablet induced apoptosis in rat HSC-T6 cells possibly in part by activating p38 and inhibiting SAPK/JNK.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Wang, Qi& Du, Hongbo& Li, Min& Li, Yue& Liu, Shunai& Gao, Ping…[et al.]. 2013. MAPK Signal Transduction Pathway Regulation : A Novel Mechanism of Rat HSC-T6 Cell Apoptosis Induced by FUZHENGHUAYU Tablet. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-13.
https://search.emarefa.net/detail/BIM-466420
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Wang, Qi…[et al.]. MAPK Signal Transduction Pathway Regulation : A Novel Mechanism of Rat HSC-T6 Cell Apoptosis Induced by FUZHENGHUAYU Tablet. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-13.
https://search.emarefa.net/detail/BIM-466420
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Wang, Qi& Du, Hongbo& Li, Min& Li, Yue& Liu, Shunai& Gao, Ping…[et al.]. MAPK Signal Transduction Pathway Regulation : A Novel Mechanism of Rat HSC-T6 Cell Apoptosis Induced by FUZHENGHUAYU Tablet. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-13.
https://search.emarefa.net/detail/BIM-466420
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-466420
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر