MAPK Signal Transduction Pathway Regulation : A Novel Mechanism of Rat HSC-T6 Cell Apoptosis Induced by FUZHENGHUAYU Tablet
Joint Authors
Li, Yue
Cheng, Jun
Zhang, Xiaoli
Du, Hongbo
Wang, Qi
Liu, Shunai
Li, Min
Gao, Ping
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-05-16
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
FUZHENGHUAYU Tablets have been widely used in the treatment of liver fibrosis in China.
Here, we investigate the apoptotic effect of FUZHENGHUAYU Tablet in rat liver stellate cell line HSC-T6.
HSC-T6 cells were incubated with control serum or drug serum from rats fed with 0.9% NaCl or FUZHENGHUAYU Tablet, respectively.
Cells exposed to drug serum showed higher proportions of early and late apoptotic cells than controls.
The mRNA levels of collagens I and III, TGF-β1 and α-SMA were reduced by drug serum compared to control serum.
Differentially expressed mRNAs and miRNAs were analyzed by microarray and sequencing, respectively.
We identified 334 differentially expressed mRNAs and also 60 GOs and two pathways related to the mRNAs.
Seventy-five differentially expressed miRNAs were down-regulated by drug serum and 1963 target genes were predicted.
134 GOs up-regulated in drug serum group were linked to miRNA targets, and drug serum also regulated 43 miRNA signal transduction pathways.
Protein levels were evaluated by Western blot.
Drug serum down-regulated (phospho-SAPK/JNK)/(SAPK/JNK) and up-regulated phospho-p38/p38 ratios.
The study showed that FUZHENGHUAYU Tablet induced apoptosis in rat HSC-T6 cells possibly in part by activating p38 and inhibiting SAPK/JNK.
American Psychological Association (APA)
Wang, Qi& Du, Hongbo& Li, Min& Li, Yue& Liu, Shunai& Gao, Ping…[et al.]. 2013. MAPK Signal Transduction Pathway Regulation : A Novel Mechanism of Rat HSC-T6 Cell Apoptosis Induced by FUZHENGHUAYU Tablet. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-13.
https://search.emarefa.net/detail/BIM-466420
Modern Language Association (MLA)
Wang, Qi…[et al.]. MAPK Signal Transduction Pathway Regulation : A Novel Mechanism of Rat HSC-T6 Cell Apoptosis Induced by FUZHENGHUAYU Tablet. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-13.
https://search.emarefa.net/detail/BIM-466420
American Medical Association (AMA)
Wang, Qi& Du, Hongbo& Li, Min& Li, Yue& Liu, Shunai& Gao, Ping…[et al.]. MAPK Signal Transduction Pathway Regulation : A Novel Mechanism of Rat HSC-T6 Cell Apoptosis Induced by FUZHENGHUAYU Tablet. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-13.
https://search.emarefa.net/detail/BIM-466420
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-466420