The Effect of Elephantopus scaber L. on Liver Regeneration after Partial Hepatectomy

الملخص EN

Liver regeneration after partial hepatectomy (PHx) is a physiological response for maintaining homeostasis.

The aim of this study is to investigate effects of Elephantopus scaber L.- (ESL-) induced liver regeneration on growth factors (HGF and IGF-1), cell cycle regulation, and apoptosis suppressed.

In this study, we fed five Chinese medicinal herbs (1 g/kg/day), Codonopsis pilosula (CP, Dangshen), Salvia miltiorrhiza Bunge (SMB, Danshen,), Bupleurum kasi (BK, Chaihu), Elephantopus scaber L.

(ESL, Teng-Khia-U), and Silymarin (Sm, 25 mg/kg) for 7 days to male Spraue-Dawley rats.

Then surgical 2/3 PHx was conducted and liver regeneration mechanisms were estimated on the following 24 hrs and 72 hrs.

The activities of growth factors (HGF and IGF-I) and cell cycle proteins were measured by Western blot and RT-PCR.

Histological analysis and apoptosis were detected by H&E stain and TUNEL.

The results showed that extraction of Elephantopus scaber L.

(ESL) and Silymarin (Sm, positive control) were increased protein expression levels of HGF and IGF-1 which leads into cell cycle.

These results suggest that the ESL plays a crucial role in cell cycle-induced liver regeneration and apoptosis.

These results suggested that the ESL plays a crucial role in cell cycle-induced liver regeneration and suppressed hepatocytes apoptosis.