Effect of Vitronectin Bound to Insulin-Like Growth Factor-I and Insulin-Like Growth Factor Binding Protein-3 on Porcine Enamel Organ-Derived Epithelial Cells
المؤلفون المشاركون
Shinohara, Yoshinori
Honda, Masaki J.
Tsuchiya, Shuhei
Hatae, Kazuo
المصدر
International Journal of Dentistry
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-04-10
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
The aim of this paper was to determine whether the interaction between IGF, IGFBP, and VN modulates the functions of porcine EOE cells.
Enamel organs from 6-month-old porcine third molars were dissociated into single epithelial cells and subcultured on culture dishes pretreated with VN, IGF-I, and IGFBP-3 (IGF-IGFBP-VN complex).
The subcultured EOE cells retained their capacity for ameloblast-related gene expression, as shown by semiquantitative reverse transcription-polymerase chain reaction.
Amelogenin expression was detected in the subcultured EOE cells by immunostaining.
The subcultured EOE cells were then seeded onto collagen sponge scaffolds in combination with fresh dental mesenchymal cells and transplanted into athymic rats.
After 4 weeks, enamel-dentin-like complex structures were present in the implanted constructs.
These results show that EOE cells cultured on IGF-IGFBP-VN complex differentiated into ameloblasts-like cells that were able to secrete amelogenin proteins and form enamel-like tissues in vivo.
Functional assays demonstrated that the IGF/IGFBP/VN complex significantly enhanced porcine EOE cell proliferation and tissue forming capacity for enamel.
This is the first study to demonstrate a functional role of the IGF-IGFBP-VN complex in EOE cells.
This application of the subculturing technique provides a foundation for further tooth-tissue engineering and for improving our understanding of ameloblast biology.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Shinohara, Yoshinori& Tsuchiya, Shuhei& Hatae, Kazuo& Honda, Masaki J.. 2012. Effect of Vitronectin Bound to Insulin-Like Growth Factor-I and Insulin-Like Growth Factor Binding Protein-3 on Porcine Enamel Organ-Derived Epithelial Cells. International Journal of Dentistry،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-468011
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Shinohara, Yoshinori…[et al.]. Effect of Vitronectin Bound to Insulin-Like Growth Factor-I and Insulin-Like Growth Factor Binding Protein-3 on Porcine Enamel Organ-Derived Epithelial Cells. International Journal of Dentistry No. 2012 (2012), pp.1-10.
https://search.emarefa.net/detail/BIM-468011
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Shinohara, Yoshinori& Tsuchiya, Shuhei& Hatae, Kazuo& Honda, Masaki J.. Effect of Vitronectin Bound to Insulin-Like Growth Factor-I and Insulin-Like Growth Factor Binding Protein-3 on Porcine Enamel Organ-Derived Epithelial Cells. International Journal of Dentistry. 2012. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-468011
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-468011
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر