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Effect of Vitronectin Bound to Insulin-Like Growth Factor-I and Insulin-Like Growth Factor Binding Protein-3 on Porcine Enamel Organ-Derived Epithelial Cells
Joint Authors
Shinohara, Yoshinori
Honda, Masaki J.
Tsuchiya, Shuhei
Hatae, Kazuo
Source
International Journal of Dentistry
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-04-10
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The aim of this paper was to determine whether the interaction between IGF, IGFBP, and VN modulates the functions of porcine EOE cells.
Enamel organs from 6-month-old porcine third molars were dissociated into single epithelial cells and subcultured on culture dishes pretreated with VN, IGF-I, and IGFBP-3 (IGF-IGFBP-VN complex).
The subcultured EOE cells retained their capacity for ameloblast-related gene expression, as shown by semiquantitative reverse transcription-polymerase chain reaction.
Amelogenin expression was detected in the subcultured EOE cells by immunostaining.
The subcultured EOE cells were then seeded onto collagen sponge scaffolds in combination with fresh dental mesenchymal cells and transplanted into athymic rats.
After 4 weeks, enamel-dentin-like complex structures were present in the implanted constructs.
These results show that EOE cells cultured on IGF-IGFBP-VN complex differentiated into ameloblasts-like cells that were able to secrete amelogenin proteins and form enamel-like tissues in vivo.
Functional assays demonstrated that the IGF/IGFBP/VN complex significantly enhanced porcine EOE cell proliferation and tissue forming capacity for enamel.
This is the first study to demonstrate a functional role of the IGF-IGFBP-VN complex in EOE cells.
This application of the subculturing technique provides a foundation for further tooth-tissue engineering and for improving our understanding of ameloblast biology.
American Psychological Association (APA)
Shinohara, Yoshinori& Tsuchiya, Shuhei& Hatae, Kazuo& Honda, Masaki J.. 2012. Effect of Vitronectin Bound to Insulin-Like Growth Factor-I and Insulin-Like Growth Factor Binding Protein-3 on Porcine Enamel Organ-Derived Epithelial Cells. International Journal of Dentistry،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-468011
Modern Language Association (MLA)
Shinohara, Yoshinori…[et al.]. Effect of Vitronectin Bound to Insulin-Like Growth Factor-I and Insulin-Like Growth Factor Binding Protein-3 on Porcine Enamel Organ-Derived Epithelial Cells. International Journal of Dentistry No. 2012 (2012), pp.1-10.
https://search.emarefa.net/detail/BIM-468011
American Medical Association (AMA)
Shinohara, Yoshinori& Tsuchiya, Shuhei& Hatae, Kazuo& Honda, Masaki J.. Effect of Vitronectin Bound to Insulin-Like Growth Factor-I and Insulin-Like Growth Factor Binding Protein-3 on Porcine Enamel Organ-Derived Epithelial Cells. International Journal of Dentistry. 2012. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-468011
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-468011