Cell Density Plays a Critical Role in Ex Vivo Expansion of T Cells for Adoptive Immunotherapy
المؤلفون المشاركون
Junghans, Richard P.
Wang, Yawen
Lo, Agnes Shuk-Yee
Ma, Qiangzhong
Gomes, Erica M.
المصدر
Journal of Biomedicine and Biotechnology
العدد
المجلد 2010، العدد 2010 (31 ديسمبر/كانون الأول 2010)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2010-06-30
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
The successful ex vivo expansion of a large numbers of T cells is a prerequisite for adoptive immunotherapy.
In this study, we found that cell density had important effects on the process of expansion of T cells in vitro.
Resting T cells were activated to expand at high cell density but failed to be activated at low cell density.
Activated T cells (ATCs) expanded rapidly at high cell density but underwent apoptosis at low cell density.
Our studies indicated that low-cell-density related ATC death is mediated by oxidative stress.
Antioxidants N-acetylcysteine, catalase, and albumin suppressed elevated reactive oxygen species (ROS) levels in low-density cultures and protected ATCs from apoptosis.
The viability of ATCs at low density was preserved by conditioned medium from high-density cultures of ATCs in which the autocrine survival factor was identified as catalase.
We also found that costimulatory signal CD28 increases T cell activation at lower cell density, paralleled by an increase in catalase secretion.
Our findings highlight the importance of cell density in T cell activation, proliferation, survival and apoptosis and support the importance of maintaining T cells at high density for their successful expansion in vitro.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Ma, Qiangzhong& Wang, Yawen& Lo, Agnes Shuk-Yee& Gomes, Erica M.& Junghans, Richard P.. 2010. Cell Density Plays a Critical Role in Ex Vivo Expansion of T Cells for Adoptive Immunotherapy. Journal of Biomedicine and Biotechnology،Vol. 2010, no. 2010, pp.1-13.
https://search.emarefa.net/detail/BIM-468042
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Ma, Qiangzhong…[et al.]. Cell Density Plays a Critical Role in Ex Vivo Expansion of T Cells for Adoptive Immunotherapy. Journal of Biomedicine and Biotechnology No. 2010 (2010), pp.1-13.
https://search.emarefa.net/detail/BIM-468042
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Ma, Qiangzhong& Wang, Yawen& Lo, Agnes Shuk-Yee& Gomes, Erica M.& Junghans, Richard P.. Cell Density Plays a Critical Role in Ex Vivo Expansion of T Cells for Adoptive Immunotherapy. Journal of Biomedicine and Biotechnology. 2010. Vol. 2010, no. 2010, pp.1-13.
https://search.emarefa.net/detail/BIM-468042
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-468042
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر