Cell Density Plays a Critical Role in Ex Vivo Expansion of T Cells for Adoptive Immunotherapy

Joint Authors

Junghans, Richard P.
Wang, Yawen
Lo, Agnes Shuk-Yee
Ma, Qiangzhong
Gomes, Erica M.

Source

Journal of Biomedicine and Biotechnology

Issue

Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-13, 13 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2010-06-30

Country of Publication

Egypt

No. of Pages

13

Main Subjects

Medicine

Abstract EN

The successful ex vivo expansion of a large numbers of T cells is a prerequisite for adoptive immunotherapy.

In this study, we found that cell density had important effects on the process of expansion of T cells in vitro.

Resting T cells were activated to expand at high cell density but failed to be activated at low cell density.

Activated T cells (ATCs) expanded rapidly at high cell density but underwent apoptosis at low cell density.

Our studies indicated that low-cell-density related ATC death is mediated by oxidative stress.

Antioxidants N-acetylcysteine, catalase, and albumin suppressed elevated reactive oxygen species (ROS) levels in low-density cultures and protected ATCs from apoptosis.

The viability of ATCs at low density was preserved by conditioned medium from high-density cultures of ATCs in which the autocrine survival factor was identified as catalase.

We also found that costimulatory signal CD28 increases T cell activation at lower cell density, paralleled by an increase in catalase secretion.

Our findings highlight the importance of cell density in T cell activation, proliferation, survival and apoptosis and support the importance of maintaining T cells at high density for their successful expansion in vitro.

American Psychological Association (APA)

Ma, Qiangzhong& Wang, Yawen& Lo, Agnes Shuk-Yee& Gomes, Erica M.& Junghans, Richard P.. 2010. Cell Density Plays a Critical Role in Ex Vivo Expansion of T Cells for Adoptive Immunotherapy. Journal of Biomedicine and Biotechnology،Vol. 2010, no. 2010, pp.1-13.
https://search.emarefa.net/detail/BIM-468042

Modern Language Association (MLA)

Ma, Qiangzhong…[et al.]. Cell Density Plays a Critical Role in Ex Vivo Expansion of T Cells for Adoptive Immunotherapy. Journal of Biomedicine and Biotechnology No. 2010 (2010), pp.1-13.
https://search.emarefa.net/detail/BIM-468042

American Medical Association (AMA)

Ma, Qiangzhong& Wang, Yawen& Lo, Agnes Shuk-Yee& Gomes, Erica M.& Junghans, Richard P.. Cell Density Plays a Critical Role in Ex Vivo Expansion of T Cells for Adoptive Immunotherapy. Journal of Biomedicine and Biotechnology. 2010. Vol. 2010, no. 2010, pp.1-13.
https://search.emarefa.net/detail/BIM-468042

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-468042