Ex Vivo Expansion of Functional Human UCB-HSCsHPCs by Coculture with AFT024-hkirre Cells
المؤلفون المشاركون
Jiao, Wei
Yousaf, Muhammad Zubair
Masood, Saima
Khan, Muti ur Rehman
Javaid, Aqeel
Feng, Meifu
Ahmad, Shafique
Wang, Yun
Ali, Ijaz
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-02-25
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Kiaa1867 (human Kirre, hKirre) has a critical role in brain development and/or maintenance of the glomerular slit diaphragm in kidneys.
Murine homolog of this gene, mKirre expressed in OP9 and AFT024 cells could support hematopoietic stem cells/hematopoietic progenitor cells (HSC/HPC) expansion in vitro.
HKirre is also expressed in human FBMOB-hTERT cell line and fetal liver fibroblast-like cells but its function has remained unclear.
In this paper, we cloned a hKirre gene from human fetal liver fibroblast-like cells and established a stably overexpressing hKirre-AFT024 cell line.
Resultant cells could promote self-renewal and ex vivo expansion of HSCs/HPCs significantly higher than AFT024-control cells transformed with mock plasmid.
The Expanded human umbilical cord blood (hUCB) CD34+ cells retained the capacity of multipotent differentiation as long as 8 weeks and successfully repopulated the bone marrow of sublethally irradiated NOD/SCID mice, which demonstrated the expansion of long-term primitive transplantable HSCs/HPCs.
Importantly, hkirre could upregulate the expressions of Wnt-5A, BMP4, and SDF-1 and downregulate TGF-β with other hematopoietic growth factors.
By SDS-PAGE and Western Blot analysis, a ~89 kDa protein in total lysate of AFT024-hKirre was identified.
Supernatants from AFT024-hkirre could also support CD34+CD38− cells expansion.
These results demonstrated that the AFT024-hKirre cells have the ability to efficiently expand HSCs/HPCs.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Khan, Muti ur Rehman& Ali, Ijaz& Jiao, Wei& Wang, Yun& Masood, Saima& Yousaf, Muhammad Zubair…[et al.]. 2014. Ex Vivo Expansion of Functional Human UCB-HSCsHPCs by Coculture with AFT024-hkirre Cells. BioMed Research International،Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-470026
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Khan, Muti ur Rehman…[et al.]. Ex Vivo Expansion of Functional Human UCB-HSCsHPCs by Coculture with AFT024-hkirre Cells. BioMed Research International No. 2014 (2014), pp.1-12.
https://search.emarefa.net/detail/BIM-470026
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Khan, Muti ur Rehman& Ali, Ijaz& Jiao, Wei& Wang, Yun& Masood, Saima& Yousaf, Muhammad Zubair…[et al.]. Ex Vivo Expansion of Functional Human UCB-HSCsHPCs by Coculture with AFT024-hkirre Cells. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-470026
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-470026
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر