Ex Vivo Expansion of Functional Human UCB-HSCsHPCs by Coculture with AFT024-hkirre Cells
Joint Authors
Jiao, Wei
Yousaf, Muhammad Zubair
Masood, Saima
Khan, Muti ur Rehman
Javaid, Aqeel
Feng, Meifu
Ahmad, Shafique
Wang, Yun
Ali, Ijaz
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-02-25
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Kiaa1867 (human Kirre, hKirre) has a critical role in brain development and/or maintenance of the glomerular slit diaphragm in kidneys.
Murine homolog of this gene, mKirre expressed in OP9 and AFT024 cells could support hematopoietic stem cells/hematopoietic progenitor cells (HSC/HPC) expansion in vitro.
HKirre is also expressed in human FBMOB-hTERT cell line and fetal liver fibroblast-like cells but its function has remained unclear.
In this paper, we cloned a hKirre gene from human fetal liver fibroblast-like cells and established a stably overexpressing hKirre-AFT024 cell line.
Resultant cells could promote self-renewal and ex vivo expansion of HSCs/HPCs significantly higher than AFT024-control cells transformed with mock plasmid.
The Expanded human umbilical cord blood (hUCB) CD34+ cells retained the capacity of multipotent differentiation as long as 8 weeks and successfully repopulated the bone marrow of sublethally irradiated NOD/SCID mice, which demonstrated the expansion of long-term primitive transplantable HSCs/HPCs.
Importantly, hkirre could upregulate the expressions of Wnt-5A, BMP4, and SDF-1 and downregulate TGF-β with other hematopoietic growth factors.
By SDS-PAGE and Western Blot analysis, a ~89 kDa protein in total lysate of AFT024-hKirre was identified.
Supernatants from AFT024-hkirre could also support CD34+CD38− cells expansion.
These results demonstrated that the AFT024-hKirre cells have the ability to efficiently expand HSCs/HPCs.
American Psychological Association (APA)
Khan, Muti ur Rehman& Ali, Ijaz& Jiao, Wei& Wang, Yun& Masood, Saima& Yousaf, Muhammad Zubair…[et al.]. 2014. Ex Vivo Expansion of Functional Human UCB-HSCsHPCs by Coculture with AFT024-hkirre Cells. BioMed Research International،Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-470026
Modern Language Association (MLA)
Khan, Muti ur Rehman…[et al.]. Ex Vivo Expansion of Functional Human UCB-HSCsHPCs by Coculture with AFT024-hkirre Cells. BioMed Research International No. 2014 (2014), pp.1-12.
https://search.emarefa.net/detail/BIM-470026
American Medical Association (AMA)
Khan, Muti ur Rehman& Ali, Ijaz& Jiao, Wei& Wang, Yun& Masood, Saima& Yousaf, Muhammad Zubair…[et al.]. Ex Vivo Expansion of Functional Human UCB-HSCsHPCs by Coculture with AFT024-hkirre Cells. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-470026
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-470026