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Proteinases in Excretory-Secretory Products of Toxocara canis Second-Stage Larvae : Zymography and Modeling Insights
المؤلفون المشاركون
González-Páez, Gonzalo Ernesto
Argüello-García, Raúl
Alba-Hurtado, Fernando
García-Tovar, Carlos Gerardo
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-08-14
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Components released in excretory-secretory products of Toxocara canis larvae (TES) include phosphatidylethanolamine-binding proteins (TES26), mucins (TES120, MUC2-5), and C-type lectins (TES32, TES70) and their biochemical, immunological, and diagnostic properties have been extensively studied albeit proteinase activities towards physiological substrates are almost unknown.
Proteolytic activities in TES samples were first analyzed by gel electrophoresis with gelatin as substrate.
Major activities of ~400, 120, and 32 kDa in TES were relatively similar over a broad pH range (5.5–9.0) and all these were of the serine-type as leupeptin abolished gelatinolysis.
Further, the ~400 kDa component degraded all physiological substrates tested (laminin, fibronectin, albumin, and goat IgG) and the 120 kDa component degraded albumin and goat IgG while proteinases of lower MW (45, 32, and 26 kDa) only degraded laminin and fibronectin, preferentially at alkaline pH (9.0).
By protein modeling approaches using the known sequences of TES components, only TES26 and MUC4 displayed folding patterns significantly related to reference serine proteinases.
These data suggest that most of serine proteinase activities secreted in vitro by infective larvae of T.
canis have intriguing nature but otherwise help the parasite to affect multiple components of somatic organs and bodily fluids within the infected host.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
González-Páez, Gonzalo Ernesto& Alba-Hurtado, Fernando& García-Tovar, Carlos Gerardo& Argüello-García, Raúl. 2014. Proteinases in Excretory-Secretory Products of Toxocara canis Second-Stage Larvae : Zymography and Modeling Insights. BioMed Research International،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-470671
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
González-Páez, Gonzalo Ernesto…[et al.]. Proteinases in Excretory-Secretory Products of Toxocara canis Second-Stage Larvae : Zymography and Modeling Insights. BioMed Research International No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-470671
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
González-Páez, Gonzalo Ernesto& Alba-Hurtado, Fernando& García-Tovar, Carlos Gerardo& Argüello-García, Raúl. Proteinases in Excretory-Secretory Products of Toxocara canis Second-Stage Larvae : Zymography and Modeling Insights. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-470671
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-470671
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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