Proteinases in Excretory-Secretory Products of Toxocara canis Second-Stage Larvae : Zymography and Modeling Insights
Joint Authors
González-Páez, Gonzalo Ernesto
Argüello-García, Raúl
Alba-Hurtado, Fernando
García-Tovar, Carlos Gerardo
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-08-14
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Components released in excretory-secretory products of Toxocara canis larvae (TES) include phosphatidylethanolamine-binding proteins (TES26), mucins (TES120, MUC2-5), and C-type lectins (TES32, TES70) and their biochemical, immunological, and diagnostic properties have been extensively studied albeit proteinase activities towards physiological substrates are almost unknown.
Proteolytic activities in TES samples were first analyzed by gel electrophoresis with gelatin as substrate.
Major activities of ~400, 120, and 32 kDa in TES were relatively similar over a broad pH range (5.5–9.0) and all these were of the serine-type as leupeptin abolished gelatinolysis.
Further, the ~400 kDa component degraded all physiological substrates tested (laminin, fibronectin, albumin, and goat IgG) and the 120 kDa component degraded albumin and goat IgG while proteinases of lower MW (45, 32, and 26 kDa) only degraded laminin and fibronectin, preferentially at alkaline pH (9.0).
By protein modeling approaches using the known sequences of TES components, only TES26 and MUC4 displayed folding patterns significantly related to reference serine proteinases.
These data suggest that most of serine proteinase activities secreted in vitro by infective larvae of T.
canis have intriguing nature but otherwise help the parasite to affect multiple components of somatic organs and bodily fluids within the infected host.
American Psychological Association (APA)
González-Páez, Gonzalo Ernesto& Alba-Hurtado, Fernando& García-Tovar, Carlos Gerardo& Argüello-García, Raúl. 2014. Proteinases in Excretory-Secretory Products of Toxocara canis Second-Stage Larvae : Zymography and Modeling Insights. BioMed Research International،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-470671
Modern Language Association (MLA)
González-Páez, Gonzalo Ernesto…[et al.]. Proteinases in Excretory-Secretory Products of Toxocara canis Second-Stage Larvae : Zymography and Modeling Insights. BioMed Research International No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-470671
American Medical Association (AMA)
González-Páez, Gonzalo Ernesto& Alba-Hurtado, Fernando& García-Tovar, Carlos Gerardo& Argüello-García, Raúl. Proteinases in Excretory-Secretory Products of Toxocara canis Second-Stage Larvae : Zymography and Modeling Insights. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-470671
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-470671