Metformin Inhibits Expression and Secretion of PEDF in Adipocyte and Hepatocyte via Promoting AMPK Phosphorylation

المؤلفون المشاركون

Lv, Qiong
Ye, Peng
Liu, Lulu
Chen, Shumei
Luo, Ting
Yang, Shumin
Cheng, Qingfeng
Gao, Rufei
Li, Qifu

المصدر

Mediators of Inflammation

العدد

المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-8، 8ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2013-10-31

دولة النشر

مصر

عدد الصفحات

8

التخصصات الرئيسية

الأمراض

الملخص EN

Objective.

Pigment epithelium-derived factor (PEDF) plays an important role in obesity-induced insulin resistance (IR).

The study aims to investigate the effect of metformin, a widely used agent to improve IR, on PEDF production both in vivo and in vitro.

Methods.

SD rats were divided into normal control group, high fat group (HF group), and metformin group (MET group).

Hyperinsulinemic euglycemic clamp was performed to evaluate insulin sensitivity.

IR models of 3T3-L1 and HepG2 cells were established and then treated with metformin and inhibitor of AMP activated protein kinase (AMPK).

Results.

In vivo, the HF group showed increased serum PEDF which is negatively correlated with insulin sensitivity, while the MET group revealed decreased serum PEDF and downregulated PEDF expression in fat and liver, concomitant with significantly improved IR.

In vitro, the IR cells showed enhanced PEDF secretion and expression, whereas metformin lowered PEDF secretion and expression, accompanied with increased glucose uptake.

Metformin stimulated AMPK phosphorylation in fat and liver of the obese rats, while in vitro, when combined with AMPK inhibitor, the effect of metformin on PEDF was abrogated.

Conclusions.

Metformin inhibits the expression and secretion of PEDF in fat and liver via promoting AMPK phosphorylation, which is closely associated with IR improvement.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Yang, Shumin& Lv, Qiong& Luo, Ting& Liu, Lulu& Gao, Rufei& Chen, Shumei…[et al.]. 2013. Metformin Inhibits Expression and Secretion of PEDF in Adipocyte and Hepatocyte via Promoting AMPK Phosphorylation. Mediators of Inflammation،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-471559

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Yang, Shumin…[et al.]. Metformin Inhibits Expression and Secretion of PEDF in Adipocyte and Hepatocyte via Promoting AMPK Phosphorylation. Mediators of Inflammation No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-471559

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Yang, Shumin& Lv, Qiong& Luo, Ting& Liu, Lulu& Gao, Rufei& Chen, Shumei…[et al.]. Metformin Inhibits Expression and Secretion of PEDF in Adipocyte and Hepatocyte via Promoting AMPK Phosphorylation. Mediators of Inflammation. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-471559

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-471559