Metformin Inhibits Expression and Secretion of PEDF in Adipocyte and Hepatocyte via Promoting AMPK Phosphorylation
Joint Authors
Lv, Qiong
Ye, Peng
Liu, Lulu
Chen, Shumei
Luo, Ting
Yang, Shumin
Cheng, Qingfeng
Gao, Rufei
Li, Qifu
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-10-31
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Objective.
Pigment epithelium-derived factor (PEDF) plays an important role in obesity-induced insulin resistance (IR).
The study aims to investigate the effect of metformin, a widely used agent to improve IR, on PEDF production both in vivo and in vitro.
Methods.
SD rats were divided into normal control group, high fat group (HF group), and metformin group (MET group).
Hyperinsulinemic euglycemic clamp was performed to evaluate insulin sensitivity.
IR models of 3T3-L1 and HepG2 cells were established and then treated with metformin and inhibitor of AMP activated protein kinase (AMPK).
Results.
In vivo, the HF group showed increased serum PEDF which is negatively correlated with insulin sensitivity, while the MET group revealed decreased serum PEDF and downregulated PEDF expression in fat and liver, concomitant with significantly improved IR.
In vitro, the IR cells showed enhanced PEDF secretion and expression, whereas metformin lowered PEDF secretion and expression, accompanied with increased glucose uptake.
Metformin stimulated AMPK phosphorylation in fat and liver of the obese rats, while in vitro, when combined with AMPK inhibitor, the effect of metformin on PEDF was abrogated.
Conclusions.
Metformin inhibits the expression and secretion of PEDF in fat and liver via promoting AMPK phosphorylation, which is closely associated with IR improvement.
American Psychological Association (APA)
Yang, Shumin& Lv, Qiong& Luo, Ting& Liu, Lulu& Gao, Rufei& Chen, Shumei…[et al.]. 2013. Metformin Inhibits Expression and Secretion of PEDF in Adipocyte and Hepatocyte via Promoting AMPK Phosphorylation. Mediators of Inflammation،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-471559
Modern Language Association (MLA)
Yang, Shumin…[et al.]. Metformin Inhibits Expression and Secretion of PEDF in Adipocyte and Hepatocyte via Promoting AMPK Phosphorylation. Mediators of Inflammation No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-471559
American Medical Association (AMA)
Yang, Shumin& Lv, Qiong& Luo, Ting& Liu, Lulu& Gao, Rufei& Chen, Shumei…[et al.]. Metformin Inhibits Expression and Secretion of PEDF in Adipocyte and Hepatocyte via Promoting AMPK Phosphorylation. Mediators of Inflammation. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-471559
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-471559