Phytic Acid Protects against 6-Hydroxydopamine-Induced Dopaminergic Neuron Apoptosis in Normal and Iron Excess Conditions in a Cell Culture Model

الملخص EN

Iron may play an important role in Parkinson's disease (PD) since it can induce oxidative stress-dependent neurodegeneration.

The objective of this study was to determine whether the iron chelator, phytic acid (IP6) can protect against 6-hydroxydopamine- (6-OHDA-) induced apoptosis in immortalized rat mesencephalic dopaminergic cells under normal and iron-excess conditions.

Caspase-3 activity was increased about 6-fold after 6-OHDA treatment (compared to control; P<.001) and 30 μmol/L IP6 pretreatment decreased it by 38% (P<.05).

Similarly, a 63% protection (P<.001) against 6-OHDA induced DNA fragmentation was observed with IP6 pretreatment.

Under iron-excess condition, a 6-fold increase in caspase-3 activity (P<.001) and a 42% increase in DNA fragmentation (P<.05) with 6-OHDA treatment were decreased by 41% (P<.01) and 27% (P<.05), respectively, with 30 μmol/L IP6.

Together, our data suggest that IP6 protects against 6-OHDA-induced cell apoptosis in both normal and iron-excess conditions, and IP6 may offer neuroprotection in PD.