Purification and Properties of Polygalacturonase Produced by Thermophilic Fungus Thermoascus aurantiacus CBMAI-756 on Solid-State Fermentation
المؤلفون المشاركون
Martins, Eduardo da Silva
Gomes, Eleni
Leite, Rodrigo Simões Ribeiro
da Silva, Roberto
المصدر
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-09-12
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
Polygalacturonases are enzymes involved in the degradation of pectic substances, being extensively used in food industries, textile processing, degumming of plant rough fibres, and treatment of pectic wastewaters.
Polygalacturonase (PG) production by thermophilic fungus Thermoascus aurantiacus on solid-state fermentation was carried out in culture media containing sugar cane bagasse and orange bagasse in proportions of 30% and 70% (w/w) at 45°C for 4 days.
PG obtained was purified by gel filtration and ion-exchange chromatography.
The highest activity was found between pH 4.5 and 5.5, and the enzyme preserved more than 80% of its activity at pH values between 5.0 and 6.5.
At pH values between 3.0 and 4.5, PG retained about 73% of the original activity, whereas at pH 10.0 it remained around 44%.
The optimum temperature was 60–65°C.
The enzyme was completely stable when incubated for 1 hour at 50°C.
At 55°C and 60°C, the activity decreased 55% and 90%, respectively.
The apparent molecular weight was 29.3 kDa, Km of 1.58 mg/mL and Vmax of 1553.1 μmol/min/mg.
The presence of Zn+2, Mn+2, and Hg+2 inhibited 59%, 77%, and 100% of enzyme activity, respectively.
The hydrolysis product suggests that polygalacturonase was shown to be an endo/exoenzyme.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Martins, Eduardo da Silva& Leite, Rodrigo Simões Ribeiro& da Silva, Roberto& Gomes, Eleni. 2013. Purification and Properties of Polygalacturonase Produced by Thermophilic Fungus Thermoascus aurantiacus CBMAI-756 on Solid-State Fermentation. Enzyme Research،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-472365
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Martins, Eduardo da Silva…[et al.]. Purification and Properties of Polygalacturonase Produced by Thermophilic Fungus Thermoascus aurantiacus CBMAI-756 on Solid-State Fermentation. Enzyme Research No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-472365
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Martins, Eduardo da Silva& Leite, Rodrigo Simões Ribeiro& da Silva, Roberto& Gomes, Eleni. Purification and Properties of Polygalacturonase Produced by Thermophilic Fungus Thermoascus aurantiacus CBMAI-756 on Solid-State Fermentation. Enzyme Research. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-472365
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-472365
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر