Purification and Properties of Polygalacturonase Produced by Thermophilic Fungus Thermoascus aurantiacus CBMAI-756 on Solid-State Fermentation
Joint Authors
Martins, Eduardo da Silva
Gomes, Eleni
Leite, Rodrigo Simões Ribeiro
da Silva, Roberto
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-09-12
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Polygalacturonases are enzymes involved in the degradation of pectic substances, being extensively used in food industries, textile processing, degumming of plant rough fibres, and treatment of pectic wastewaters.
Polygalacturonase (PG) production by thermophilic fungus Thermoascus aurantiacus on solid-state fermentation was carried out in culture media containing sugar cane bagasse and orange bagasse in proportions of 30% and 70% (w/w) at 45°C for 4 days.
PG obtained was purified by gel filtration and ion-exchange chromatography.
The highest activity was found between pH 4.5 and 5.5, and the enzyme preserved more than 80% of its activity at pH values between 5.0 and 6.5.
At pH values between 3.0 and 4.5, PG retained about 73% of the original activity, whereas at pH 10.0 it remained around 44%.
The optimum temperature was 60–65°C.
The enzyme was completely stable when incubated for 1 hour at 50°C.
At 55°C and 60°C, the activity decreased 55% and 90%, respectively.
The apparent molecular weight was 29.3 kDa, Km of 1.58 mg/mL and Vmax of 1553.1 μmol/min/mg.
The presence of Zn+2, Mn+2, and Hg+2 inhibited 59%, 77%, and 100% of enzyme activity, respectively.
The hydrolysis product suggests that polygalacturonase was shown to be an endo/exoenzyme.
American Psychological Association (APA)
Martins, Eduardo da Silva& Leite, Rodrigo Simões Ribeiro& da Silva, Roberto& Gomes, Eleni. 2013. Purification and Properties of Polygalacturonase Produced by Thermophilic Fungus Thermoascus aurantiacus CBMAI-756 on Solid-State Fermentation. Enzyme Research،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-472365
Modern Language Association (MLA)
Martins, Eduardo da Silva…[et al.]. Purification and Properties of Polygalacturonase Produced by Thermophilic Fungus Thermoascus aurantiacus CBMAI-756 on Solid-State Fermentation. Enzyme Research No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-472365
American Medical Association (AMA)
Martins, Eduardo da Silva& Leite, Rodrigo Simões Ribeiro& da Silva, Roberto& Gomes, Eleni. Purification and Properties of Polygalacturonase Produced by Thermophilic Fungus Thermoascus aurantiacus CBMAI-756 on Solid-State Fermentation. Enzyme Research. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-472365
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-472365