A Noncellulosomal Mannanase26E Contains a CBM59 in Clostridium cellulovorans
المؤلفون المشاركون
Yamamoto, Kosuke
Tamaru, Yutaka
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-03-27
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
A multicomponent enzyme-complex prevents efficient degradation of the plant cell wall for biorefinery.
In this study, the method of identifying glycoside hydrolases (GHs) to degrade hemicelluloses was demonstrated.
The competence of C.
cellulovorans, which changes to be suitable for degradation of each carbon source, was used for the method.
C.
cellulovorans was cultivated into locust bean gum (LBG) that is composed of galactomannan.
The proteins produced by C.
cellulovorans were separated into either fractions binding to crystalline cellulose or not.
Proteins obtained from each fraction were further separated by SDS-PAGE and were stained with Coomassie Brilliant Blue and were detected for mannanase activity.
The proteins having the enzymatic activity for LBG were cut out and were identified by mass spectrometry.
As a result, four protein bands were classified into glycosyl hydrolase family 26 (GH26) mannanases.
One of the identified mannanases, Man26E, contains a carbohydrate-binding module (CBM) family 59, which binds to xylan, mannan, and Avicel.
Although mannose and galactose are the same as a hexose, the expression patterns of the proteins from C.
cellulovorans were quite different.
More interestingly, zymogram for mannanase activity showed that Man26E was detected in only LBG medium.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Yamamoto, Kosuke& Tamaru, Yutaka. 2014. A Noncellulosomal Mannanase26E Contains a CBM59 in Clostridium cellulovorans. BioMed Research International،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-472385
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Yamamoto, Kosuke& Tamaru, Yutaka. A Noncellulosomal Mannanase26E Contains a CBM59 in Clostridium cellulovorans. BioMed Research International No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-472385
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Yamamoto, Kosuke& Tamaru, Yutaka. A Noncellulosomal Mannanase26E Contains a CBM59 in Clostridium cellulovorans. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-472385
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-472385
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر