A Noncellulosomal Mannanase26E Contains a CBM59 in Clostridium cellulovorans
Joint Authors
Yamamoto, Kosuke
Tamaru, Yutaka
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-03-27
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
A multicomponent enzyme-complex prevents efficient degradation of the plant cell wall for biorefinery.
In this study, the method of identifying glycoside hydrolases (GHs) to degrade hemicelluloses was demonstrated.
The competence of C.
cellulovorans, which changes to be suitable for degradation of each carbon source, was used for the method.
C.
cellulovorans was cultivated into locust bean gum (LBG) that is composed of galactomannan.
The proteins produced by C.
cellulovorans were separated into either fractions binding to crystalline cellulose or not.
Proteins obtained from each fraction were further separated by SDS-PAGE and were stained with Coomassie Brilliant Blue and were detected for mannanase activity.
The proteins having the enzymatic activity for LBG were cut out and were identified by mass spectrometry.
As a result, four protein bands were classified into glycosyl hydrolase family 26 (GH26) mannanases.
One of the identified mannanases, Man26E, contains a carbohydrate-binding module (CBM) family 59, which binds to xylan, mannan, and Avicel.
Although mannose and galactose are the same as a hexose, the expression patterns of the proteins from C.
cellulovorans were quite different.
More interestingly, zymogram for mannanase activity showed that Man26E was detected in only LBG medium.
American Psychological Association (APA)
Yamamoto, Kosuke& Tamaru, Yutaka. 2014. A Noncellulosomal Mannanase26E Contains a CBM59 in Clostridium cellulovorans. BioMed Research International،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-472385
Modern Language Association (MLA)
Yamamoto, Kosuke& Tamaru, Yutaka. A Noncellulosomal Mannanase26E Contains a CBM59 in Clostridium cellulovorans. BioMed Research International No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-472385
American Medical Association (AMA)
Yamamoto, Kosuke& Tamaru, Yutaka. A Noncellulosomal Mannanase26E Contains a CBM59 in Clostridium cellulovorans. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-472385
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-472385