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PI3KAkt Pathway Contributes to Neurovascular Unit Protection of Xiao-Xu-Ming Decoction against Focal Cerebral Ischemia and Reperfusion Injury in Rats
المؤلفون المشاركون
Lan, Rui
Bao, Jie
Li, Wen-Wei
Wang, Guo-Hua
Xiang, Jun
Xu, Li-Li
Cai, Ding-Fang
Zhang, Yong
Zhang, Wen
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-16، 16ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-05-28
دولة النشر
مصر
عدد الصفحات
16
التخصصات الرئيسية
الملخص EN
In the present study, we used a focal cerebral ischemia and reperfusion rat model to investigate the protective effects of Xiao-Xu-Ming decoction (XXMD) on neurovascular unit and to examine the role of PI3K (phosphatidylinositol 3-kinase)/Akt pathway in this protection.
The cerebral ischemia was induced by 90 min of middle cerebral artery occlusion.
Cerebral infarct area was measured by tetrazolium staining, and neurological function was observed at 24 h after reperfusion.
DNA fragmentation assay, combined with immunofluorescence, was performed to evaluate apoptosis of neuron, astrocyte, and vascular endothelial cell which constitute neurovascular unit.
The expression levels of proteins involved in PI3K/Akt pathway were detected by Western blot.
The results showed that XXMD improved neurological function, decreased cerebral infarct area and neuronal damage, and attenuated cellular apoptosis in neurovascular unit, while these effects were abolished by inhibition of PI3K/Akt with LY294002.
We also found that XXMD upregulated p-PDKl, p-Akt, and p-GSK3β expression levels, which were partly reversed by LY294002.
In addition, the increases of p-PTEN and p-c-Raf expression levels on which LY294002 had no effect were also observed in response to XXMD treatment.
The data indicated the protective effects of XXMD on neurovascular unit partly through the activation of PI3K/Akt pathway.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Lan, Rui& Xiang, Jun& Zhang, Yong& Wang, Guo-Hua& Bao, Jie& Li, Wen-Wei…[et al.]. 2013. PI3KAkt Pathway Contributes to Neurovascular Unit Protection of Xiao-Xu-Ming Decoction against Focal Cerebral Ischemia and Reperfusion Injury in Rats. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-16.
https://search.emarefa.net/detail/BIM-473228
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Lan, Rui…[et al.]. PI3KAkt Pathway Contributes to Neurovascular Unit Protection of Xiao-Xu-Ming Decoction against Focal Cerebral Ischemia and Reperfusion Injury in Rats. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-16.
https://search.emarefa.net/detail/BIM-473228
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Lan, Rui& Xiang, Jun& Zhang, Yong& Wang, Guo-Hua& Bao, Jie& Li, Wen-Wei…[et al.]. PI3KAkt Pathway Contributes to Neurovascular Unit Protection of Xiao-Xu-Ming Decoction against Focal Cerebral Ischemia and Reperfusion Injury in Rats. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-16.
https://search.emarefa.net/detail/BIM-473228
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-473228
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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