PI3KAkt Pathway Contributes to Neurovascular Unit Protection of Xiao-Xu-Ming Decoction against Focal Cerebral Ischemia and Reperfusion Injury in Rats
Joint Authors
Lan, Rui
Bao, Jie
Li, Wen-Wei
Wang, Guo-Hua
Xiang, Jun
Xu, Li-Li
Cai, Ding-Fang
Zhang, Yong
Zhang, Wen
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-16, 16 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-05-28
Country of Publication
Egypt
No. of Pages
16
Main Subjects
Abstract EN
In the present study, we used a focal cerebral ischemia and reperfusion rat model to investigate the protective effects of Xiao-Xu-Ming decoction (XXMD) on neurovascular unit and to examine the role of PI3K (phosphatidylinositol 3-kinase)/Akt pathway in this protection.
The cerebral ischemia was induced by 90 min of middle cerebral artery occlusion.
Cerebral infarct area was measured by tetrazolium staining, and neurological function was observed at 24 h after reperfusion.
DNA fragmentation assay, combined with immunofluorescence, was performed to evaluate apoptosis of neuron, astrocyte, and vascular endothelial cell which constitute neurovascular unit.
The expression levels of proteins involved in PI3K/Akt pathway were detected by Western blot.
The results showed that XXMD improved neurological function, decreased cerebral infarct area and neuronal damage, and attenuated cellular apoptosis in neurovascular unit, while these effects were abolished by inhibition of PI3K/Akt with LY294002.
We also found that XXMD upregulated p-PDKl, p-Akt, and p-GSK3β expression levels, which were partly reversed by LY294002.
In addition, the increases of p-PTEN and p-c-Raf expression levels on which LY294002 had no effect were also observed in response to XXMD treatment.
The data indicated the protective effects of XXMD on neurovascular unit partly through the activation of PI3K/Akt pathway.
American Psychological Association (APA)
Lan, Rui& Xiang, Jun& Zhang, Yong& Wang, Guo-Hua& Bao, Jie& Li, Wen-Wei…[et al.]. 2013. PI3KAkt Pathway Contributes to Neurovascular Unit Protection of Xiao-Xu-Ming Decoction against Focal Cerebral Ischemia and Reperfusion Injury in Rats. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-16.
https://search.emarefa.net/detail/BIM-473228
Modern Language Association (MLA)
Lan, Rui…[et al.]. PI3KAkt Pathway Contributes to Neurovascular Unit Protection of Xiao-Xu-Ming Decoction against Focal Cerebral Ischemia and Reperfusion Injury in Rats. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-16.
https://search.emarefa.net/detail/BIM-473228
American Medical Association (AMA)
Lan, Rui& Xiang, Jun& Zhang, Yong& Wang, Guo-Hua& Bao, Jie& Li, Wen-Wei…[et al.]. PI3KAkt Pathway Contributes to Neurovascular Unit Protection of Xiao-Xu-Ming Decoction against Focal Cerebral Ischemia and Reperfusion Injury in Rats. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-16.
https://search.emarefa.net/detail/BIM-473228
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-473228