Inhibition of Neuroinflammation in LPS-Activated Microglia by Cryptolepine
المؤلفون المشاركون
Wright, Colin W.
Olajide, Olumayokun A.
de Oliveira, Antonio C. P.
Fiebich, Bernd L.
Bhatia, Harsharan S.
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-05-08
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Cryptolepine, an indoloquinoline alkaloid in Cryptolepis sanguinolenta, has anti-inflammatory property.
In this study, we aimed to evaluate the effects of cryptolepine on lipopolysaccharide (LPS)- induced neuroinflammation in rat microglia and its potential mechanisms.
Microglial activation was induced by stimulation with LPS, and the effects of cryptolepine pretreatment on microglial activation and production of proinflammatory mediators, PGE2/COX-2, microsomal prostaglandin E2 synthase and nitric oxide/iNOS were investigated.
We further elucidated the role of Nuclear Factor-kappa B (NF-κB) and the mitogen-activated protein kinases in the antiinflammatory actions of cryptolepine in LPS-stimulated microglia.
Our results showed that cryptolepine significantly inhibited LPS-induced production of tumour necrosis factor-alpha (TNFα), interleukin-6 (IL-6), interleukin-1beta (IL-1β), nitric oxide, and PGE2.
Protein and mRNA levels of COX-2 and iNOS were also attenuated by cryptolepine.
Further experiments on intracellular signalling mechanisms show that IκB-independent inhibition of NF-κB nuclear translocation contributes to the anti-neuroinflammatory actions of cryptolepine.
Results also show that cryptolepine inhibited LPS-induced p38 and MAPKAPK2 phosphorylation in the microglia.
Cell viability experiments revealed that cryptolepine (2.5 and 5 μM) did not produce cytotoxicity in microglia.
Taken together, our results suggest that cryptolepine inhibits LPS-induced microglial inflammation by partial targeting of NF-κB signalling and attenuation of p38/MAPKAPK2.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Olajide, Olumayokun A.& Bhatia, Harsharan S.& de Oliveira, Antonio C. P.& Wright, Colin W.& Fiebich, Bernd L.. 2013. Inhibition of Neuroinflammation in LPS-Activated Microglia by Cryptolepine. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-473249
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Olajide, Olumayokun A.…[et al.]. Inhibition of Neuroinflammation in LPS-Activated Microglia by Cryptolepine. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-473249
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Olajide, Olumayokun A.& Bhatia, Harsharan S.& de Oliveira, Antonio C. P.& Wright, Colin W.& Fiebich, Bernd L.. Inhibition of Neuroinflammation in LPS-Activated Microglia by Cryptolepine. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-473249
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-473249
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر