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Inhibition of Neuroinflammation in LPS-Activated Microglia by Cryptolepine
Joint Authors
Wright, Colin W.
Olajide, Olumayokun A.
de Oliveira, Antonio C. P.
Fiebich, Bernd L.
Bhatia, Harsharan S.
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-05-08
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Cryptolepine, an indoloquinoline alkaloid in Cryptolepis sanguinolenta, has anti-inflammatory property.
In this study, we aimed to evaluate the effects of cryptolepine on lipopolysaccharide (LPS)- induced neuroinflammation in rat microglia and its potential mechanisms.
Microglial activation was induced by stimulation with LPS, and the effects of cryptolepine pretreatment on microglial activation and production of proinflammatory mediators, PGE2/COX-2, microsomal prostaglandin E2 synthase and nitric oxide/iNOS were investigated.
We further elucidated the role of Nuclear Factor-kappa B (NF-κB) and the mitogen-activated protein kinases in the antiinflammatory actions of cryptolepine in LPS-stimulated microglia.
Our results showed that cryptolepine significantly inhibited LPS-induced production of tumour necrosis factor-alpha (TNFα), interleukin-6 (IL-6), interleukin-1beta (IL-1β), nitric oxide, and PGE2.
Protein and mRNA levels of COX-2 and iNOS were also attenuated by cryptolepine.
Further experiments on intracellular signalling mechanisms show that IκB-independent inhibition of NF-κB nuclear translocation contributes to the anti-neuroinflammatory actions of cryptolepine.
Results also show that cryptolepine inhibited LPS-induced p38 and MAPKAPK2 phosphorylation in the microglia.
Cell viability experiments revealed that cryptolepine (2.5 and 5 μM) did not produce cytotoxicity in microglia.
Taken together, our results suggest that cryptolepine inhibits LPS-induced microglial inflammation by partial targeting of NF-κB signalling and attenuation of p38/MAPKAPK2.
American Psychological Association (APA)
Olajide, Olumayokun A.& Bhatia, Harsharan S.& de Oliveira, Antonio C. P.& Wright, Colin W.& Fiebich, Bernd L.. 2013. Inhibition of Neuroinflammation in LPS-Activated Microglia by Cryptolepine. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-473249
Modern Language Association (MLA)
Olajide, Olumayokun A.…[et al.]. Inhibition of Neuroinflammation in LPS-Activated Microglia by Cryptolepine. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-473249
American Medical Association (AMA)
Olajide, Olumayokun A.& Bhatia, Harsharan S.& de Oliveira, Antonio C. P.& Wright, Colin W.& Fiebich, Bernd L.. Inhibition of Neuroinflammation in LPS-Activated Microglia by Cryptolepine. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-473249
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-473249