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Polymorphism of Myostatin Gene in Intron 1 and 2 and Exon 3, and Their Associations with Yearling Weight, Using PCR-RFLP and PCR-SSCP Techniques in Zel Sheep
المؤلفون المشاركون
Mohajer, Mokhtar
Khan Ahmadi, Alireza
Hasani, Saeed
Ahani Azari, Mojtaba
Yousefi, Soheil
Shahmohamadi, Leila
Dehnavi, Elena
Nassiry, Mohammad Reza
المصدر
Biotechnology Research International
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-5، 5ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-06-20
دولة النشر
مصر
عدد الصفحات
5
التخصصات الرئيسية
تكنولوجيا المعلومات وعلم الحاسوب
الملخص EN
The aim of present study was to investigate myostatin gene polymorphism and its association with yearling weight records in Zel sheep using PCR-RFLP and PCR-SSCP methods.
Blood samples were collected from 200 Zel sheep, randomly, and DNA was extracted using modified salting out method.
Polymerase chain reaction was carried out to amplify 337, 222, and 311 bp fragments, respectively, comprising a part of exon 3, intron 1, and intron 2 of myostatin gene.
In addition, exon 3 was digested by HaeIII enzyme under RFLP method, and introns 1 and 2 were studied using SSCP.
Under RFLP method, all samples showed mm genotype.
Under SSCP method, intron 1 was also monomorph but intron 2 was polymorph (AA, AB, and BB).
The allelic frequencies for A and B were 75.5 and 24.5%, respectively.
This locus was not in Hardy-Weinberg equilibrium (P<0.05), and there was no significant effect of myostatin gene on yearling weights.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Dehnavi, Elena& Ahani Azari, Mojtaba& Hasani, Saeed& Nassiry, Mohammad Reza& Mohajer, Mokhtar& Khan Ahmadi, Alireza…[et al.]. 2012. Polymorphism of Myostatin Gene in Intron 1 and 2 and Exon 3, and Their Associations with Yearling Weight, Using PCR-RFLP and PCR-SSCP Techniques in Zel Sheep. Biotechnology Research International،Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-474218
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Dehnavi, Elena…[et al.]. Polymorphism of Myostatin Gene in Intron 1 and 2 and Exon 3, and Their Associations with Yearling Weight, Using PCR-RFLP and PCR-SSCP Techniques in Zel Sheep. Biotechnology Research International No. 2012 (2012), pp.1-5.
https://search.emarefa.net/detail/BIM-474218
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Dehnavi, Elena& Ahani Azari, Mojtaba& Hasani, Saeed& Nassiry, Mohammad Reza& Mohajer, Mokhtar& Khan Ahmadi, Alireza…[et al.]. Polymorphism of Myostatin Gene in Intron 1 and 2 and Exon 3, and Their Associations with Yearling Weight, Using PCR-RFLP and PCR-SSCP Techniques in Zel Sheep. Biotechnology Research International. 2012. Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-474218
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-474218
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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