Polymorphism of Myostatin Gene in Intron 1 and 2 and Exon 3, and Their Associations with Yearling Weight, Using PCR-RFLP and PCR-SSCP Techniques in Zel Sheep
Joint Authors
Mohajer, Mokhtar
Khan Ahmadi, Alireza
Hasani, Saeed
Ahani Azari, Mojtaba
Yousefi, Soheil
Shahmohamadi, Leila
Dehnavi, Elena
Nassiry, Mohammad Reza
Source
Biotechnology Research International
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-06-20
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Information Technology and Computer Science
Abstract EN
The aim of present study was to investigate myostatin gene polymorphism and its association with yearling weight records in Zel sheep using PCR-RFLP and PCR-SSCP methods.
Blood samples were collected from 200 Zel sheep, randomly, and DNA was extracted using modified salting out method.
Polymerase chain reaction was carried out to amplify 337, 222, and 311 bp fragments, respectively, comprising a part of exon 3, intron 1, and intron 2 of myostatin gene.
In addition, exon 3 was digested by HaeIII enzyme under RFLP method, and introns 1 and 2 were studied using SSCP.
Under RFLP method, all samples showed mm genotype.
Under SSCP method, intron 1 was also monomorph but intron 2 was polymorph (AA, AB, and BB).
The allelic frequencies for A and B were 75.5 and 24.5%, respectively.
This locus was not in Hardy-Weinberg equilibrium (P<0.05), and there was no significant effect of myostatin gene on yearling weights.
American Psychological Association (APA)
Dehnavi, Elena& Ahani Azari, Mojtaba& Hasani, Saeed& Nassiry, Mohammad Reza& Mohajer, Mokhtar& Khan Ahmadi, Alireza…[et al.]. 2012. Polymorphism of Myostatin Gene in Intron 1 and 2 and Exon 3, and Their Associations with Yearling Weight, Using PCR-RFLP and PCR-SSCP Techniques in Zel Sheep. Biotechnology Research International،Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-474218
Modern Language Association (MLA)
Dehnavi, Elena…[et al.]. Polymorphism of Myostatin Gene in Intron 1 and 2 and Exon 3, and Their Associations with Yearling Weight, Using PCR-RFLP and PCR-SSCP Techniques in Zel Sheep. Biotechnology Research International No. 2012 (2012), pp.1-5.
https://search.emarefa.net/detail/BIM-474218
American Medical Association (AMA)
Dehnavi, Elena& Ahani Azari, Mojtaba& Hasani, Saeed& Nassiry, Mohammad Reza& Mohajer, Mokhtar& Khan Ahmadi, Alireza…[et al.]. Polymorphism of Myostatin Gene in Intron 1 and 2 and Exon 3, and Their Associations with Yearling Weight, Using PCR-RFLP and PCR-SSCP Techniques in Zel Sheep. Biotechnology Research International. 2012. Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-474218
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-474218