Direct Spectrophotometric Assay for Benzaldehyde Lyase Activity

الملخص EN

Benzaldehyde lyase from Pseudomonas fluorescens Biovar I.

(BAL, EC 4.1.2.38) is a versatile catalyst for the organic synthesis of chiral α-hydroxy ketones.

To allow fast assessment of enzyme activity, a direct spectrophotometric assay is desirable.

Here, a new robust and easy-to-handle assay based on UV absorption is presented.

The assay developed is based on the ligation of the α-hydroxy ketone (R)-2,2′-furoin from 2-furaldehyde.

A robust assay with direct monitoring of the product is facilitated with a convenient concentration working range minimising experimental associated with low concentrations.