Direct Spectrophotometric Assay for Benzaldehyde Lyase Activity
Joint Authors
Ansorge-Schumacher, Marion B.
Natalia, Dessy
Greiner, Lasse
Kohlmann, Christina
Source
Biotechnology Research International
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-4, 4 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-07-14
Country of Publication
Egypt
No. of Pages
4
Main Subjects
Information Technology and Computer Science
Abstract EN
Benzaldehyde lyase from Pseudomonas fluorescens Biovar I.
(BAL, EC 4.1.2.38) is a versatile catalyst for the organic synthesis of chiral α-hydroxy ketones.
To allow fast assessment of enzyme activity, a direct spectrophotometric assay is desirable.
Here, a new robust and easy-to-handle assay based on UV absorption is presented.
The assay developed is based on the ligation of the α-hydroxy ketone (R)-2,2′-furoin from 2-furaldehyde.
A robust assay with direct monitoring of the product is facilitated with a convenient concentration working range minimising experimental associated with low concentrations.
American Psychological Association (APA)
Natalia, Dessy& Kohlmann, Christina& Ansorge-Schumacher, Marion B.& Greiner, Lasse. 2011. Direct Spectrophotometric Assay for Benzaldehyde Lyase Activity. Biotechnology Research International،Vol. 2011, no. 2011, pp.1-4.
https://search.emarefa.net/detail/BIM-474760
Modern Language Association (MLA)
Natalia, Dessy…[et al.]. Direct Spectrophotometric Assay for Benzaldehyde Lyase Activity. Biotechnology Research International No. 2011 (2011), pp.1-4.
https://search.emarefa.net/detail/BIM-474760
American Medical Association (AMA)
Natalia, Dessy& Kohlmann, Christina& Ansorge-Schumacher, Marion B.& Greiner, Lasse. Direct Spectrophotometric Assay for Benzaldehyde Lyase Activity. Biotechnology Research International. 2011. Vol. 2011, no. 2011, pp.1-4.
https://search.emarefa.net/detail/BIM-474760
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-474760
