Synthesis and G-Quadruplex-Binding Properties of Defined Acridine Oligomers

الملخص EN

The synthesis of oligomers containing two or three acridine units linked through 2-aminoethylglycine using solid-phase methodology is described.

Subsequent studies on cell viability showed that these compounds are not cytotoxic.

Binding to several DNA structures was studied by competitive dialysis, which showed a clear affinity for DNA sequences that form G-quadruplexes and parallel triplexes.

The fluorescence spectra of acridine oligomers were affected strongly upon binding to DNA.

These spectral changes were used to calculate the binding constants (K).

Log K were found to be in the order of 4–6.