Novel Molecular Markers of Malignancy in Histologically Normal and Benign Breast
المؤلفون المشاركون
Henderson-Jackson, Evita B.
Chen, Dung-Tsa
McCarthy, Susan M.
McBride, Heyoung L.
Khakpour, Nazanin
Gruidl, Mike
Venkataramu, Chinnambally
Nasir, Aejaz
Yeatman, Timothy J.
Harris, Eleanor E.
المصدر
Pathology Research International
العدد
المجلد 2011، العدد 2011 (31 ديسمبر/كانون الأول 2011)، ص ص. 1-18، 18ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2011-07-10
دولة النشر
مصر
عدد الصفحات
18
التخصصات الرئيسية
الملخص EN
To detect the molecular changes of malignancy in histologically normal breast (HNB) tissues, we recently developed a novel 117-gene-malignancy-signature.
Here we report validation of our leading malignancy-risk-genes, topoisomerase-2-alpha (TOP2A), minichromosome-maintenance-protein-2 (MCM2) and “budding-uninhibited-by-benzimidazoles-1-homolog-beta” (BUB1B) at the protein level.
Using our 117-gene malignancy-signature, we classified 18 fresh-frozen HNB tissues from 18 adult female breast cancer patients into HNB-tissues with low-grade (HNB-LGMA; N=9) and high-grade molecular abnormality (HNB-HGMA; N=9).
Archival sections of additional HNB tissues from these patients, and invasive ductal carcinoma (IDC) tissues from six other patients were immunostained for these biomarkers.
TOP2A/MCM2 expression was assessed as staining index (%) and BUB1B expression as H-scores (0–300).
Increasing TOP2A, MCM2, and BUB1B protein expression from HNB-LGMA to HNB-HGMA tissues to IDCs validated our microarray-based molecular classification of HNB tissues by immunohistochemistry.
We also demonstrated an increasing expression of TOP2A protein on an independent test set of HNB/benign/reductionmammoplasties, atypical-ductal-hyperplasia with and without synchronous breast cancer, DCIS and IDC tissues using a custom tissue microarray (TMA).
In conclusion, TOP2A, MCM2, and BUB1B proteins are potential molecular biomarkers of malignancy in histologically normal and benign breast tissues.
Larger-scale clinical validation studies are needed to further evaluate the clinical utility of these molecular biomarkers.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Nasir, Aejaz& McCarthy, Susan M.& McBride, Heyoung L.& Harris, Eleanor E.& Khakpour, Nazanin& Yeatman, Timothy J.…[et al.]. 2011. Novel Molecular Markers of Malignancy in Histologically Normal and Benign Breast. Pathology Research International،Vol. 2011, no. 2011, pp.1-18.
https://search.emarefa.net/detail/BIM-475639
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
McCarthy, Susan M.…[et al.]. Novel Molecular Markers of Malignancy in Histologically Normal and Benign Breast. Pathology Research International No. 2011 (2011), pp.1-18.
https://search.emarefa.net/detail/BIM-475639
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Nasir, Aejaz& McCarthy, Susan M.& McBride, Heyoung L.& Harris, Eleanor E.& Khakpour, Nazanin& Yeatman, Timothy J.…[et al.]. Novel Molecular Markers of Malignancy in Histologically Normal and Benign Breast. Pathology Research International. 2011. Vol. 2011, no. 2011, pp.1-18.
https://search.emarefa.net/detail/BIM-475639
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-475639
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر