Novel Molecular Markers of Malignancy in Histologically Normal and Benign Breast
Joint Authors
Henderson-Jackson, Evita B.
Chen, Dung-Tsa
McCarthy, Susan M.
McBride, Heyoung L.
Khakpour, Nazanin
Gruidl, Mike
Venkataramu, Chinnambally
Nasir, Aejaz
Yeatman, Timothy J.
Harris, Eleanor E.
Source
Pathology Research International
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-18, 18 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-07-10
Country of Publication
Egypt
No. of Pages
18
Main Subjects
Abstract EN
To detect the molecular changes of malignancy in histologically normal breast (HNB) tissues, we recently developed a novel 117-gene-malignancy-signature.
Here we report validation of our leading malignancy-risk-genes, topoisomerase-2-alpha (TOP2A), minichromosome-maintenance-protein-2 (MCM2) and “budding-uninhibited-by-benzimidazoles-1-homolog-beta” (BUB1B) at the protein level.
Using our 117-gene malignancy-signature, we classified 18 fresh-frozen HNB tissues from 18 adult female breast cancer patients into HNB-tissues with low-grade (HNB-LGMA; N=9) and high-grade molecular abnormality (HNB-HGMA; N=9).
Archival sections of additional HNB tissues from these patients, and invasive ductal carcinoma (IDC) tissues from six other patients were immunostained for these biomarkers.
TOP2A/MCM2 expression was assessed as staining index (%) and BUB1B expression as H-scores (0–300).
Increasing TOP2A, MCM2, and BUB1B protein expression from HNB-LGMA to HNB-HGMA tissues to IDCs validated our microarray-based molecular classification of HNB tissues by immunohistochemistry.
We also demonstrated an increasing expression of TOP2A protein on an independent test set of HNB/benign/reductionmammoplasties, atypical-ductal-hyperplasia with and without synchronous breast cancer, DCIS and IDC tissues using a custom tissue microarray (TMA).
In conclusion, TOP2A, MCM2, and BUB1B proteins are potential molecular biomarkers of malignancy in histologically normal and benign breast tissues.
Larger-scale clinical validation studies are needed to further evaluate the clinical utility of these molecular biomarkers.
American Psychological Association (APA)
Nasir, Aejaz& McCarthy, Susan M.& McBride, Heyoung L.& Harris, Eleanor E.& Khakpour, Nazanin& Yeatman, Timothy J.…[et al.]. 2011. Novel Molecular Markers of Malignancy in Histologically Normal and Benign Breast. Pathology Research International،Vol. 2011, no. 2011, pp.1-18.
https://search.emarefa.net/detail/BIM-475639
Modern Language Association (MLA)
McCarthy, Susan M.…[et al.]. Novel Molecular Markers of Malignancy in Histologically Normal and Benign Breast. Pathology Research International No. 2011 (2011), pp.1-18.
https://search.emarefa.net/detail/BIM-475639
American Medical Association (AMA)
Nasir, Aejaz& McCarthy, Susan M.& McBride, Heyoung L.& Harris, Eleanor E.& Khakpour, Nazanin& Yeatman, Timothy J.…[et al.]. Novel Molecular Markers of Malignancy in Histologically Normal and Benign Breast. Pathology Research International. 2011. Vol. 2011, no. 2011, pp.1-18.
https://search.emarefa.net/detail/BIM-475639
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-475639