Th17 : A New Participant in Gut Dysfunction in Mice Infected with Trichinella spiralis

الملخص EN

Trichinella spiralis infection in rodents is a well-known model of intestinal inflammation associated with hypermotility.

Our aim was to elucidate if Th17 cells were involved in the development of gastrointestinal hypermotility in this experimental model.

Intestinal inflammation was observed by hematoxylin-eosin (HE) staining.

Jejunal smooth muscle contractility was investigated in response to acetylcholine (Ach).

The effects of IL-17 on jejunum smooth muscle contractility were explored.

Flow cytometry was used to analyze the proportion of Th17 cells in jejunum.

The levels of IL-17, IL-23, and TGF-β1 in jejunum were measured by Western blot.

Our results showed that the inflammation in jejunum was severe at 2 weeks postinfection (PI), which was not discernible at 8 weeks PI.

Jejunal smooth muscle contractility was increased at 2 weeks PI and kept higher at 12 weeks PI.

The proportion of Th17 cells and the expression of IL-17 were upregulated in jejunum at 2 weeks PI and normalized at 8 weeks PI.

When jejunual smooth muscle strips were cultured with IL-17, contractions elicited by Ach were enhanced in a concentration-dependent manner.

Our data suggest that Th17 cells are increased during acute infection with Trichinella spiralis and IL-17 may contribute to jejunal muscle contractility in mice.