Th17 : A New Participant in Gut Dysfunction in Mice Infected with Trichinella spiralis
Joint Authors
Wang, Wenfeng
Qian, Wei
Pan, Qi
Long, Yanqing
Hou, Xiaohua
Fu, Yu
Tong, Jingjing
Source
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2009-11-18
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Trichinella spiralis infection in rodents is a well-known model of intestinal inflammation associated with hypermotility.
Our aim was to elucidate if Th17 cells were involved in the development of gastrointestinal hypermotility in this experimental model.
Intestinal inflammation was observed by hematoxylin-eosin (HE) staining.
Jejunal smooth muscle contractility was investigated in response to acetylcholine (Ach).
The effects of IL-17 on jejunum smooth muscle contractility were explored.
Flow cytometry was used to analyze the proportion of Th17 cells in jejunum.
The levels of IL-17, IL-23, and TGF-β1 in jejunum were measured by Western blot.
Our results showed that the inflammation in jejunum was severe at 2 weeks postinfection (PI), which was not discernible at 8 weeks PI.
Jejunal smooth muscle contractility was increased at 2 weeks PI and kept higher at 12 weeks PI.
The proportion of Th17 cells and the expression of IL-17 were upregulated in jejunum at 2 weeks PI and normalized at 8 weeks PI.
When jejunual smooth muscle strips were cultured with IL-17, contractions elicited by Ach were enhanced in a concentration-dependent manner.
Our data suggest that Th17 cells are increased during acute infection with Trichinella spiralis and IL-17 may contribute to jejunal muscle contractility in mice.
American Psychological Association (APA)
Fu, Yu& Wang, Wenfeng& Tong, Jingjing& Pan, Qi& Long, Yanqing& Qian, Wei…[et al.]. 2009. Th17 : A New Participant in Gut Dysfunction in Mice Infected with Trichinella spiralis. Mediators of Inflammation،Vol. 2009, no. 2009, pp.1-7.
https://search.emarefa.net/detail/BIM-477943
Modern Language Association (MLA)
Fu, Yu…[et al.]. Th17 : A New Participant in Gut Dysfunction in Mice Infected with Trichinella spiralis. Mediators of Inflammation No. 2009 (2009), pp.1-7.
https://search.emarefa.net/detail/BIM-477943
American Medical Association (AMA)
Fu, Yu& Wang, Wenfeng& Tong, Jingjing& Pan, Qi& Long, Yanqing& Qian, Wei…[et al.]. Th17 : A New Participant in Gut Dysfunction in Mice Infected with Trichinella spiralis. Mediators of Inflammation. 2009. Vol. 2009, no. 2009, pp.1-7.
https://search.emarefa.net/detail/BIM-477943
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-477943