Effect of Hyaluronan on Developmental Competence and Quality of Oocytes and Obtained Blastocysts from In Vitro Maturation of Bovine Oocytes
المؤلفون المشاركون
Opiela, Jolanta
Romanek, Joanna
Lipiński, Daniel
Smorąg, Zdzisław
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-02-13
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
The objective of the present study was to evaluate the effect of hyaluronan (HA) during IVM on meiotic maturation, embryonic development, and the quality of oocytes, granulosa cells (GC), and obtained blastocysts.
COCs were matured in vitro in control medium and medium with additional 0.035% or 0.07% of exogenous HA.
The meiotic maturity did not differ between the analysed groups.
The best rate and the highest quality of obtained blastocysts were observed when 0.07% HA was used.
A highly significant difference (P<0.001) was noted in the mean number of apoptotic nuclei per blastocyst and in the DCI between the 0.07% HA and the control blastocysts (P<0.01).
Our results suggest that addition of 0.035% HA and 0.07% HA to oocyte maturation media does not affect oocyte nuclear maturation and DNA fragmentation.
However, the addition of 0.07% HA during IVM decreases the level of blastocysts DNA fragmentation.
Finally, our results suggest that it may be risky to increase the HA concentration during IVM above 0.07% as we found significantly higher Bax mRNA expression levels in GC cultured with 0.07% HA.
The final concentration of HA being supplemented to oocyte maturation media is critical for the success of the IVP procedure.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Opiela, Jolanta& Romanek, Joanna& Lipiński, Daniel& Smorąg, Zdzisław. 2014. Effect of Hyaluronan on Developmental Competence and Quality of Oocytes and Obtained Blastocysts from In Vitro Maturation of Bovine Oocytes. BioMed Research International،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-478139
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Opiela, Jolanta…[et al.]. Effect of Hyaluronan on Developmental Competence and Quality of Oocytes and Obtained Blastocysts from In Vitro Maturation of Bovine Oocytes. BioMed Research International No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-478139
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Opiela, Jolanta& Romanek, Joanna& Lipiński, Daniel& Smorąg, Zdzisław. Effect of Hyaluronan on Developmental Competence and Quality of Oocytes and Obtained Blastocysts from In Vitro Maturation of Bovine Oocytes. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-478139
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-478139
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر