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Effect of Hyaluronan on Developmental Competence and Quality of Oocytes and Obtained Blastocysts from In Vitro Maturation of Bovine Oocytes
Joint Authors
Opiela, Jolanta
Romanek, Joanna
Lipiński, Daniel
Smorąg, Zdzisław
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-02-13
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
The objective of the present study was to evaluate the effect of hyaluronan (HA) during IVM on meiotic maturation, embryonic development, and the quality of oocytes, granulosa cells (GC), and obtained blastocysts.
COCs were matured in vitro in control medium and medium with additional 0.035% or 0.07% of exogenous HA.
The meiotic maturity did not differ between the analysed groups.
The best rate and the highest quality of obtained blastocysts were observed when 0.07% HA was used.
A highly significant difference (P<0.001) was noted in the mean number of apoptotic nuclei per blastocyst and in the DCI between the 0.07% HA and the control blastocysts (P<0.01).
Our results suggest that addition of 0.035% HA and 0.07% HA to oocyte maturation media does not affect oocyte nuclear maturation and DNA fragmentation.
However, the addition of 0.07% HA during IVM decreases the level of blastocysts DNA fragmentation.
Finally, our results suggest that it may be risky to increase the HA concentration during IVM above 0.07% as we found significantly higher Bax mRNA expression levels in GC cultured with 0.07% HA.
The final concentration of HA being supplemented to oocyte maturation media is critical for the success of the IVP procedure.
American Psychological Association (APA)
Opiela, Jolanta& Romanek, Joanna& Lipiński, Daniel& Smorąg, Zdzisław. 2014. Effect of Hyaluronan on Developmental Competence and Quality of Oocytes and Obtained Blastocysts from In Vitro Maturation of Bovine Oocytes. BioMed Research International،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-478139
Modern Language Association (MLA)
Opiela, Jolanta…[et al.]. Effect of Hyaluronan on Developmental Competence and Quality of Oocytes and Obtained Blastocysts from In Vitro Maturation of Bovine Oocytes. BioMed Research International No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-478139
American Medical Association (AMA)
Opiela, Jolanta& Romanek, Joanna& Lipiński, Daniel& Smorąg, Zdzisław. Effect of Hyaluronan on Developmental Competence and Quality of Oocytes and Obtained Blastocysts from In Vitro Maturation of Bovine Oocytes. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-478139
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-478139