Mannosylated Chitosan Nanoparticles for Delivery of Antisense Oligonucleotides for Macrophage Targeting
المؤلفون المشاركون
Shilakari Asthana, Gyati
Asthana, Abhay
Kohli, Dharm Veer
Vyas, Suresh Prasad
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-17، 17ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-06-26
دولة النشر
مصر
عدد الصفحات
17
التخصصات الرئيسية
الملخص EN
The therapeutic potential of antisense oligonucleotides (ASODN) is primarily dependent upon its safe and efficient delivery to specific cells overcoming degradation and maximizing cellular uptake in vivo.
The present study focuses on designing mannosylated low molecular weight (LMW) chitosan nanoconstructs for safe ODNs delivery by macrophage targeting.
Mannose groups were coupled with LMW chitosan and characterized spectroscopically.
Mannosylated chitosan ODN nanoparticles (MCHODN NPs) were formulated by self-assembled method using various N/P ratio (moles of amine groups of MCH to phosphate moieties of ODNs) and characterized for gel retardation assay, physicochemical characteristics, cytotoxicity and transfection efficiency, and antisense assay.
Complete complexation of MCH/ODN was achieved at charge ratio of 1:1 and above.
On increasing the N/P ratio of MCH/ODN, particle size of the NPs decreased whereas zeta potential (ZV) increased.
MCHODN NPs displayed much higher transfection efficiency into Raw 264.7 cells (bears mannose receptors) than Hela cells and no significant toxicity was observed at all MCH concentrations.
Antisense assay revealed that reduction in lipopolysaccharide (LPS) induced serum TNF-α is due to antisense activity of TJU-2755 ODN (sequence complementary to 3′-UTR of TNF-α).
These results suggest that MCHODN NPs are acceptable choice to improve transfection efficiency in vitro and in vivo.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Shilakari Asthana, Gyati& Asthana, Abhay& Kohli, Dharm Veer& Vyas, Suresh Prasad. 2014. Mannosylated Chitosan Nanoparticles for Delivery of Antisense Oligonucleotides for Macrophage Targeting. BioMed Research International،Vol. 2014, no. 2014, pp.1-17.
https://search.emarefa.net/detail/BIM-478708
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Shilakari Asthana, Gyati…[et al.]. Mannosylated Chitosan Nanoparticles for Delivery of Antisense Oligonucleotides for Macrophage Targeting. BioMed Research International No. 2014 (2014), pp.1-17.
https://search.emarefa.net/detail/BIM-478708
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Shilakari Asthana, Gyati& Asthana, Abhay& Kohli, Dharm Veer& Vyas, Suresh Prasad. Mannosylated Chitosan Nanoparticles for Delivery of Antisense Oligonucleotides for Macrophage Targeting. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-17.
https://search.emarefa.net/detail/BIM-478708
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-478708
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر