Mannosylated Chitosan Nanoparticles for Delivery of Antisense Oligonucleotides for Macrophage Targeting
Joint Authors
Shilakari Asthana, Gyati
Asthana, Abhay
Kohli, Dharm Veer
Vyas, Suresh Prasad
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-17, 17 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-06-26
Country of Publication
Egypt
No. of Pages
17
Main Subjects
Abstract EN
The therapeutic potential of antisense oligonucleotides (ASODN) is primarily dependent upon its safe and efficient delivery to specific cells overcoming degradation and maximizing cellular uptake in vivo.
The present study focuses on designing mannosylated low molecular weight (LMW) chitosan nanoconstructs for safe ODNs delivery by macrophage targeting.
Mannose groups were coupled with LMW chitosan and characterized spectroscopically.
Mannosylated chitosan ODN nanoparticles (MCHODN NPs) were formulated by self-assembled method using various N/P ratio (moles of amine groups of MCH to phosphate moieties of ODNs) and characterized for gel retardation assay, physicochemical characteristics, cytotoxicity and transfection efficiency, and antisense assay.
Complete complexation of MCH/ODN was achieved at charge ratio of 1:1 and above.
On increasing the N/P ratio of MCH/ODN, particle size of the NPs decreased whereas zeta potential (ZV) increased.
MCHODN NPs displayed much higher transfection efficiency into Raw 264.7 cells (bears mannose receptors) than Hela cells and no significant toxicity was observed at all MCH concentrations.
Antisense assay revealed that reduction in lipopolysaccharide (LPS) induced serum TNF-α is due to antisense activity of TJU-2755 ODN (sequence complementary to 3′-UTR of TNF-α).
These results suggest that MCHODN NPs are acceptable choice to improve transfection efficiency in vitro and in vivo.
American Psychological Association (APA)
Shilakari Asthana, Gyati& Asthana, Abhay& Kohli, Dharm Veer& Vyas, Suresh Prasad. 2014. Mannosylated Chitosan Nanoparticles for Delivery of Antisense Oligonucleotides for Macrophage Targeting. BioMed Research International،Vol. 2014, no. 2014, pp.1-17.
https://search.emarefa.net/detail/BIM-478708
Modern Language Association (MLA)
Shilakari Asthana, Gyati…[et al.]. Mannosylated Chitosan Nanoparticles for Delivery of Antisense Oligonucleotides for Macrophage Targeting. BioMed Research International No. 2014 (2014), pp.1-17.
https://search.emarefa.net/detail/BIM-478708
American Medical Association (AMA)
Shilakari Asthana, Gyati& Asthana, Abhay& Kohli, Dharm Veer& Vyas, Suresh Prasad. Mannosylated Chitosan Nanoparticles for Delivery of Antisense Oligonucleotides for Macrophage Targeting. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-17.
https://search.emarefa.net/detail/BIM-478708
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-478708