Synthetic Nucleotides as Probes of DNA Polymerase Specificity

المؤلفون المشاركون

Beuning, Penny J.
Walsh, Jason M.

المصدر

Journal of Nucleic Acids

العدد

المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-17، 17ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2012-06-07

دولة النشر

مصر

عدد الصفحات

17

التخصصات الرئيسية

الأحياء
الطب البشري

الملخص EN

The genetic code is continuously expanding with new nucleobases designed to suit specific research needs.

These synthetic nucleotides are used to study DNA polymerase dynamics and specificity and may even inhibit DNA polymerase activity.

The availability of an increasing chemical diversity of nucleotides allows questions of utilization by different DNA polymerases to be addressed.

Much of the work in this area deals with the A family DNA polymerases, for example, Escherichia coli DNA polymerase I, which are DNA polymerases involved in replication and whose fidelity is relatively high, but more recent work includes other families of polymerases, including the Y family, whose members are known to be error prone.

This paper focuses on the ability of DNA polymerases to utilize nonnatural nucleotides in DNA templates or as the incoming nucleoside triphosphates.

Beyond the utility of nonnatural nucleotides as probes of DNA polymerase specificity, such entities can also provide insight into the functions of DNA polymerases when encountering DNA that is damaged by natural agents.

Thus, synthetic nucleotides provide insight into how polymerases deal with nonnatural nucleotides as well as into the mutagenic potential of nonnatural nucleotides.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Walsh, Jason M.& Beuning, Penny J.. 2012. Synthetic Nucleotides as Probes of DNA Polymerase Specificity. Journal of Nucleic Acids،Vol. 2012, no. 2012, pp.1-17.
https://search.emarefa.net/detail/BIM-479134

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Walsh, Jason M.& Beuning, Penny J.. Synthetic Nucleotides as Probes of DNA Polymerase Specificity. Journal of Nucleic Acids No. 2012 (2012), pp.1-17.
https://search.emarefa.net/detail/BIM-479134

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Walsh, Jason M.& Beuning, Penny J.. Synthetic Nucleotides as Probes of DNA Polymerase Specificity. Journal of Nucleic Acids. 2012. Vol. 2012, no. 2012, pp.1-17.
https://search.emarefa.net/detail/BIM-479134

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-479134