Synthetic Nucleotides as Probes of DNA Polymerase Specificity
Joint Authors
Beuning, Penny J.
Walsh, Jason M.
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-17, 17 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-06-07
Country of Publication
Egypt
No. of Pages
17
Main Subjects
Abstract EN
The genetic code is continuously expanding with new nucleobases designed to suit specific research needs.
These synthetic nucleotides are used to study DNA polymerase dynamics and specificity and may even inhibit DNA polymerase activity.
The availability of an increasing chemical diversity of nucleotides allows questions of utilization by different DNA polymerases to be addressed.
Much of the work in this area deals with the A family DNA polymerases, for example, Escherichia coli DNA polymerase I, which are DNA polymerases involved in replication and whose fidelity is relatively high, but more recent work includes other families of polymerases, including the Y family, whose members are known to be error prone.
This paper focuses on the ability of DNA polymerases to utilize nonnatural nucleotides in DNA templates or as the incoming nucleoside triphosphates.
Beyond the utility of nonnatural nucleotides as probes of DNA polymerase specificity, such entities can also provide insight into the functions of DNA polymerases when encountering DNA that is damaged by natural agents.
Thus, synthetic nucleotides provide insight into how polymerases deal with nonnatural nucleotides as well as into the mutagenic potential of nonnatural nucleotides.
American Psychological Association (APA)
Walsh, Jason M.& Beuning, Penny J.. 2012. Synthetic Nucleotides as Probes of DNA Polymerase Specificity. Journal of Nucleic Acids،Vol. 2012, no. 2012, pp.1-17.
https://search.emarefa.net/detail/BIM-479134
Modern Language Association (MLA)
Walsh, Jason M.& Beuning, Penny J.. Synthetic Nucleotides as Probes of DNA Polymerase Specificity. Journal of Nucleic Acids No. 2012 (2012), pp.1-17.
https://search.emarefa.net/detail/BIM-479134
American Medical Association (AMA)
Walsh, Jason M.& Beuning, Penny J.. Synthetic Nucleotides as Probes of DNA Polymerase Specificity. Journal of Nucleic Acids. 2012. Vol. 2012, no. 2012, pp.1-17.
https://search.emarefa.net/detail/BIM-479134
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-479134