The In Vivo Antidiabetic Activity of Nigella sativa Is Mediated through Activation of the AMPK Pathway and Increased Muscle Glut4 Content

المؤلفون المشاركون

Settaf, Abdellatif
Martineau, Louis C.
Haddad, Pierre S.
Madiraju, Padma
Vuong, Tri
Meddah, Bouchra
Benhaddou-Andaloussi, Ali

المصدر

Evidence-Based Complementary and Alternative Medicine

العدد

المجلد 2011، العدد 2011 (31 ديسمبر/كانون الأول 2011)، ص ص. 1-9، 9ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2011-04-14

دولة النشر

مصر

عدد الصفحات

9

التخصصات الرئيسية

الطب البشري

الملخص EN

The antidiabetic effect of N.

sativa seed ethanol extract (NSE) was assessed in Meriones shawi after development of diabetes.

Meriones shawi were divided randomly into four groups: normal control, diabetic control, diabetic treated with NSE (2 g eq plant/kg) or with metformin (300 mg/kg) positive control, both administered by daily intragastric gavage for 4 weeks.

Glycaemia and body weight were evaluated weekly.

At study's end, an Oral Glucose Tolerance Test (OGTT) was performed to estimate insulin sensitivity.

Upon sacrifice, plasma lipid profile, insulin, leptin, and adiponectin levels were assessed.

ACC phosphorylation and Glut4 protein content were determined in liver and skeletal muscle.

NSE animals showed a progressive normalization of glycaemia, albeit slower than that of metformin controls.

Moreover, NSE increased insulinemia and HDL-cholesterol, compared to diabetic controls.

Leptin and adiponectin were unchanged.

NSE treatment decreased OGTT and tended to decrease liver and muscle triglyceride content.

NSE stimulated muscle and liver ACC phosphorylation and increased muscle Glut4.

These results confirm NSE's previously reported hypoglycaemic and hypolipidemic activity.

More significantly, our data demonstrate that in vivo treatment with NSE exerts an insulin-sensitizing action by enhancing ACC phosphorylation, a major component of the insulin-independent AMPK signaling pathway, and by enhancing muscle Glut4 expression.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Benhaddou-Andaloussi, Ali& Martineau, Louis C.& Vuong, Tri& Meddah, Bouchra& Madiraju, Padma& Settaf, Abdellatif…[et al.]. 2011. The In Vivo Antidiabetic Activity of Nigella sativa Is Mediated through Activation of the AMPK Pathway and Increased Muscle Glut4 Content. Evidence-Based Complementary and Alternative Medicine،Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-479730

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Benhaddou-Andaloussi, Ali…[et al.]. The In Vivo Antidiabetic Activity of Nigella sativa Is Mediated through Activation of the AMPK Pathway and Increased Muscle Glut4 Content. Evidence-Based Complementary and Alternative Medicine No. 2011 (2011), pp.1-9.
https://search.emarefa.net/detail/BIM-479730

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Benhaddou-Andaloussi, Ali& Martineau, Louis C.& Vuong, Tri& Meddah, Bouchra& Madiraju, Padma& Settaf, Abdellatif…[et al.]. The In Vivo Antidiabetic Activity of Nigella sativa Is Mediated through Activation of the AMPK Pathway and Increased Muscle Glut4 Content. Evidence-Based Complementary and Alternative Medicine. 2011. Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-479730

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-479730