Destruxin B Isolated from Entomopathogenic Fungus Metarhizium anisopliae Induces Apoptosis via a Bcl-2 Family-Dependent Mitochondrial Pathway in Human Nonsmall Cell Lung Cancer Cells
المؤلفون المشاركون
Liu, Bing-Lan
Tzeng, Yew-Min
Chen, Tzu-Hsiu
Wu, Chun-Chi
Hsu, Shih-Lan
Chen, Yung-Ching
Wu, Li-Chen
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-09-24
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
Destruxin B, isolated from entomopathogenic fungus Metarhizium anisopliae, is one of the cyclodepsipeptides with insecticidal and anticancer activities.
In this study, destruxin B was extracted and purified by ion-exchange chromatography, silica gel chromatography, and semipreparative high-performance liquid chromatography.
The potential anticancer effects and molecular mechanisms of destruxin B in human nonsmall cell lung cancer cell lines were characterized.
Our results showed that destruxin B induced apoptotic cell death in A549 cells.
This event was accompanied by the activation of caspase-2, -3, and -9.
Moreover, destruxin B increased the expression level of proapoptotic molecule, PUMA, while decreased antiapoptotic molecule Mcl-1.
Additionally, the translocation of Bax from cytosol to mitochondrial membrane was observed upon destruxin B treatment.
Knockdown of Bax by shRNA effectively attenuated destruxin-B-triggered apoptosis in A549 cells.
Interestingly, similar toxic effects and underlying mechanisms including caspase activation, upregulation of PUMA, and downregulation of Mcl-1 were also observed in a p53-null lung cancer H1299 cell line upon destruxin B treatment.
Taken together, our findings suggest that destruxin-B-induced apoptosis in human nonsmall cell lung cancer cells is via a Bcl-2 family-dependent mitochondrial pathway.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Wu, Chun-Chi& Chen, Tzu-Hsiu& Liu, Bing-Lan& Wu, Li-Chen& Chen, Yung-Ching& Tzeng, Yew-Min…[et al.]. 2013. Destruxin B Isolated from Entomopathogenic Fungus Metarhizium anisopliae Induces Apoptosis via a Bcl-2 Family-Dependent Mitochondrial Pathway in Human Nonsmall Cell Lung Cancer Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-480605
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Wu, Chun-Chi…[et al.]. Destruxin B Isolated from Entomopathogenic Fungus Metarhizium anisopliae Induces Apoptosis via a Bcl-2 Family-Dependent Mitochondrial Pathway in Human Nonsmall Cell Lung Cancer Cells. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-11.
https://search.emarefa.net/detail/BIM-480605
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Wu, Chun-Chi& Chen, Tzu-Hsiu& Liu, Bing-Lan& Wu, Li-Chen& Chen, Yung-Ching& Tzeng, Yew-Min…[et al.]. Destruxin B Isolated from Entomopathogenic Fungus Metarhizium anisopliae Induces Apoptosis via a Bcl-2 Family-Dependent Mitochondrial Pathway in Human Nonsmall Cell Lung Cancer Cells. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-480605
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-480605
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر