Destruxin B Isolated from Entomopathogenic Fungus Metarhizium anisopliae Induces Apoptosis via a Bcl-2 Family-Dependent Mitochondrial Pathway in Human Nonsmall Cell Lung Cancer Cells
Joint Authors
Liu, Bing-Lan
Tzeng, Yew-Min
Chen, Tzu-Hsiu
Wu, Chun-Chi
Hsu, Shih-Lan
Chen, Yung-Ching
Wu, Li-Chen
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-09-24
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Destruxin B, isolated from entomopathogenic fungus Metarhizium anisopliae, is one of the cyclodepsipeptides with insecticidal and anticancer activities.
In this study, destruxin B was extracted and purified by ion-exchange chromatography, silica gel chromatography, and semipreparative high-performance liquid chromatography.
The potential anticancer effects and molecular mechanisms of destruxin B in human nonsmall cell lung cancer cell lines were characterized.
Our results showed that destruxin B induced apoptotic cell death in A549 cells.
This event was accompanied by the activation of caspase-2, -3, and -9.
Moreover, destruxin B increased the expression level of proapoptotic molecule, PUMA, while decreased antiapoptotic molecule Mcl-1.
Additionally, the translocation of Bax from cytosol to mitochondrial membrane was observed upon destruxin B treatment.
Knockdown of Bax by shRNA effectively attenuated destruxin-B-triggered apoptosis in A549 cells.
Interestingly, similar toxic effects and underlying mechanisms including caspase activation, upregulation of PUMA, and downregulation of Mcl-1 were also observed in a p53-null lung cancer H1299 cell line upon destruxin B treatment.
Taken together, our findings suggest that destruxin-B-induced apoptosis in human nonsmall cell lung cancer cells is via a Bcl-2 family-dependent mitochondrial pathway.
American Psychological Association (APA)
Wu, Chun-Chi& Chen, Tzu-Hsiu& Liu, Bing-Lan& Wu, Li-Chen& Chen, Yung-Ching& Tzeng, Yew-Min…[et al.]. 2013. Destruxin B Isolated from Entomopathogenic Fungus Metarhizium anisopliae Induces Apoptosis via a Bcl-2 Family-Dependent Mitochondrial Pathway in Human Nonsmall Cell Lung Cancer Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-480605
Modern Language Association (MLA)
Wu, Chun-Chi…[et al.]. Destruxin B Isolated from Entomopathogenic Fungus Metarhizium anisopliae Induces Apoptosis via a Bcl-2 Family-Dependent Mitochondrial Pathway in Human Nonsmall Cell Lung Cancer Cells. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-11.
https://search.emarefa.net/detail/BIM-480605
American Medical Association (AMA)
Wu, Chun-Chi& Chen, Tzu-Hsiu& Liu, Bing-Lan& Wu, Li-Chen& Chen, Yung-Ching& Tzeng, Yew-Min…[et al.]. Destruxin B Isolated from Entomopathogenic Fungus Metarhizium anisopliae Induces Apoptosis via a Bcl-2 Family-Dependent Mitochondrial Pathway in Human Nonsmall Cell Lung Cancer Cells. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-480605
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-480605