Conversion to Sirolimus Ameliorates Cyclosporine-Induced Nephropathy in the Rat : Focus on Serum, Urine, Gene, and Protein Renal Expression Biomarkers
المؤلفون المشاركون
Teixeira, Frederico
Vala, Helena
Sereno, José
Fernandes, João
Rocha-Pereira, Petronila
Rodrigues-Santos, Paulo
Nunes, Sara
Santos-Silva, Alice
Reis, Flávio
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-17، 17ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-05-19
دولة النشر
مصر
عدد الصفحات
17
التخصصات الرئيسية
الملخص EN
Protocols of conversion from cyclosporin A (CsA) to sirolimus (SRL) have been widely used in immunotherapy after transplantation to prevent CsA-induced nephropathy, but the molecular mechanisms underlying these protocols remain nuclear.
This study aimed to identify the molecular pathways and putative biomarkers of CsA-to-SRL conversion in a rat model.
Four animal groups (n=6) were tested during 9 weeks: control, CsA, SRL, and conversion (CsA for 3 weeks followed by SRL for 6 weeks).
Classical and emergent serum, urinary, and kidney tissue (gene and protein expression) markers were assessed.
Renal lesions were analyzed in hematoxylin and eosin, periodic acid-Schiff, and Masson’s trichrome stains.
SRL-treated rats presented proteinuria and NGAL (serum and urinary) as the best markers of renal impairment.
Short CsA treatment presented slight or even absent kidney lesions and TGF-β, NF-κβ, mTOR, PCNA, TP53, KIM-1, and CTGF as relevant gene and protein changes.
Prolonged CsA exposure aggravated renal damage, without clear changes on the traditional markers, but with changes in serums TGF-β and IL-7, TBARs clearance, and kidney TGF-β and mTOR.
Conversion to SRL prevented CsA-induced renal damage evolution (absent/mild grade lesions), while NGAL (serum versus urine) seems to be a feasible biomarker of CsA replacement to SRL.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Sereno, José& Nunes, Sara& Rodrigues-Santos, Paulo& Vala, Helena& Rocha-Pereira, Petronila& Fernandes, João…[et al.]. 2014. Conversion to Sirolimus Ameliorates Cyclosporine-Induced Nephropathy in the Rat : Focus on Serum, Urine, Gene, and Protein Renal Expression Biomarkers. BioMed Research International،Vol. 2014, no. 2014, pp.1-17.
https://search.emarefa.net/detail/BIM-482161
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Sereno, José…[et al.]. Conversion to Sirolimus Ameliorates Cyclosporine-Induced Nephropathy in the Rat : Focus on Serum, Urine, Gene, and Protein Renal Expression Biomarkers. BioMed Research International No. 2014 (2014), pp.1-17.
https://search.emarefa.net/detail/BIM-482161
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Sereno, José& Nunes, Sara& Rodrigues-Santos, Paulo& Vala, Helena& Rocha-Pereira, Petronila& Fernandes, João…[et al.]. Conversion to Sirolimus Ameliorates Cyclosporine-Induced Nephropathy in the Rat : Focus on Serum, Urine, Gene, and Protein Renal Expression Biomarkers. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-17.
https://search.emarefa.net/detail/BIM-482161
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-482161
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر