Conversion to Sirolimus Ameliorates Cyclosporine-Induced Nephropathy in the Rat : Focus on Serum, Urine, Gene, and Protein Renal Expression Biomarkers
Joint Authors
Teixeira, Frederico
Vala, Helena
Sereno, José
Fernandes, João
Rocha-Pereira, Petronila
Rodrigues-Santos, Paulo
Nunes, Sara
Santos-Silva, Alice
Reis, Flávio
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-17, 17 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-19
Country of Publication
Egypt
No. of Pages
17
Main Subjects
Abstract EN
Protocols of conversion from cyclosporin A (CsA) to sirolimus (SRL) have been widely used in immunotherapy after transplantation to prevent CsA-induced nephropathy, but the molecular mechanisms underlying these protocols remain nuclear.
This study aimed to identify the molecular pathways and putative biomarkers of CsA-to-SRL conversion in a rat model.
Four animal groups (n=6) were tested during 9 weeks: control, CsA, SRL, and conversion (CsA for 3 weeks followed by SRL for 6 weeks).
Classical and emergent serum, urinary, and kidney tissue (gene and protein expression) markers were assessed.
Renal lesions were analyzed in hematoxylin and eosin, periodic acid-Schiff, and Masson’s trichrome stains.
SRL-treated rats presented proteinuria and NGAL (serum and urinary) as the best markers of renal impairment.
Short CsA treatment presented slight or even absent kidney lesions and TGF-β, NF-κβ, mTOR, PCNA, TP53, KIM-1, and CTGF as relevant gene and protein changes.
Prolonged CsA exposure aggravated renal damage, without clear changes on the traditional markers, but with changes in serums TGF-β and IL-7, TBARs clearance, and kidney TGF-β and mTOR.
Conversion to SRL prevented CsA-induced renal damage evolution (absent/mild grade lesions), while NGAL (serum versus urine) seems to be a feasible biomarker of CsA replacement to SRL.
American Psychological Association (APA)
Sereno, José& Nunes, Sara& Rodrigues-Santos, Paulo& Vala, Helena& Rocha-Pereira, Petronila& Fernandes, João…[et al.]. 2014. Conversion to Sirolimus Ameliorates Cyclosporine-Induced Nephropathy in the Rat : Focus on Serum, Urine, Gene, and Protein Renal Expression Biomarkers. BioMed Research International،Vol. 2014, no. 2014, pp.1-17.
https://search.emarefa.net/detail/BIM-482161
Modern Language Association (MLA)
Sereno, José…[et al.]. Conversion to Sirolimus Ameliorates Cyclosporine-Induced Nephropathy in the Rat : Focus on Serum, Urine, Gene, and Protein Renal Expression Biomarkers. BioMed Research International No. 2014 (2014), pp.1-17.
https://search.emarefa.net/detail/BIM-482161
American Medical Association (AMA)
Sereno, José& Nunes, Sara& Rodrigues-Santos, Paulo& Vala, Helena& Rocha-Pereira, Petronila& Fernandes, João…[et al.]. Conversion to Sirolimus Ameliorates Cyclosporine-Induced Nephropathy in the Rat : Focus on Serum, Urine, Gene, and Protein Renal Expression Biomarkers. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-17.
https://search.emarefa.net/detail/BIM-482161
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-482161