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BSA Nanoparticles for siRNA Delivery : Coating Effects on Nanoparticle Properties, Plasma Protein Adsorption, and In Vitro siRNA Delivery
المؤلفون المشاركون
Unsworth, Larry David
Singh, Harsh-Deep
Aliabadi, Hamidreza Montezari
Yogasundaram, Haran
Uludağ, Hasan
Bahniuk, Markian Stephan
المصدر
International Journal of Biomaterials
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-08-07
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Developing vehicles for the delivery of therapeutic molecules, like siRNA, is an area of active research.
Nanoparticles composed of bovine serum albumin, stabilized via the adsorption of poly-L-lysine (PLL), have been shown to be potentially inert drug-delivery vehicles.
With the primary goal of reducing nonspecific protein adsorption, the effect of using comb-type structures of poly(ethylene glycol) (1 kDa, PEG) units conjugated to PLL (4.2 and 24 kDa) on BSA-NP properties, apparent siRNA release rate, cell viability, and cell uptake were evaluated.
PEGylated PLL coatings resulted in NPs with ζ-potentials close to neutral.
Incubation with platelet-poor plasma showed the composition of the adsorbed proteome was similar for all systems.
siRNA was effectively encapsulated and released in a sustained manner from all NPs.
With 4.2 kDa PLL, cellular uptake was not affected by the presence of PEG, but PEG coating inhibited uptake with 24 kDa PLL NPs.
Moreover, 24 kDa PLL systems were cytotoxic and this cytotoxicity was diminished upon PEG incorporation.
The overall results identified a BSA-NP coating structure that provided effective siRNA encapsulation while reducing ζ-potential, protein adsorption, and cytotoxicity, necessary attributes for in vivo application of drug-delivery vehicles.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Yogasundaram, Haran& Bahniuk, Markian Stephan& Singh, Harsh-Deep& Aliabadi, Hamidreza Montezari& Uludağ, Hasan& Unsworth, Larry David. 2012. BSA Nanoparticles for siRNA Delivery : Coating Effects on Nanoparticle Properties, Plasma Protein Adsorption, and In Vitro siRNA Delivery. International Journal of Biomaterials،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-482732
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Yogasundaram, Haran…[et al.]. BSA Nanoparticles for siRNA Delivery : Coating Effects on Nanoparticle Properties, Plasma Protein Adsorption, and In Vitro siRNA Delivery. International Journal of Biomaterials No. 2012 (2012), pp.1-10.
https://search.emarefa.net/detail/BIM-482732
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Yogasundaram, Haran& Bahniuk, Markian Stephan& Singh, Harsh-Deep& Aliabadi, Hamidreza Montezari& Uludağ, Hasan& Unsworth, Larry David. BSA Nanoparticles for siRNA Delivery : Coating Effects on Nanoparticle Properties, Plasma Protein Adsorption, and In Vitro siRNA Delivery. International Journal of Biomaterials. 2012. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-482732
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-482732
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
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