BSA Nanoparticles for siRNA Delivery : Coating Effects on Nanoparticle Properties, Plasma Protein Adsorption, and In Vitro siRNA Delivery
Joint Authors
Unsworth, Larry David
Singh, Harsh-Deep
Aliabadi, Hamidreza Montezari
Yogasundaram, Haran
Uludağ, Hasan
Bahniuk, Markian Stephan
Source
International Journal of Biomaterials
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-08-07
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Developing vehicles for the delivery of therapeutic molecules, like siRNA, is an area of active research.
Nanoparticles composed of bovine serum albumin, stabilized via the adsorption of poly-L-lysine (PLL), have been shown to be potentially inert drug-delivery vehicles.
With the primary goal of reducing nonspecific protein adsorption, the effect of using comb-type structures of poly(ethylene glycol) (1 kDa, PEG) units conjugated to PLL (4.2 and 24 kDa) on BSA-NP properties, apparent siRNA release rate, cell viability, and cell uptake were evaluated.
PEGylated PLL coatings resulted in NPs with ζ-potentials close to neutral.
Incubation with platelet-poor plasma showed the composition of the adsorbed proteome was similar for all systems.
siRNA was effectively encapsulated and released in a sustained manner from all NPs.
With 4.2 kDa PLL, cellular uptake was not affected by the presence of PEG, but PEG coating inhibited uptake with 24 kDa PLL NPs.
Moreover, 24 kDa PLL systems were cytotoxic and this cytotoxicity was diminished upon PEG incorporation.
The overall results identified a BSA-NP coating structure that provided effective siRNA encapsulation while reducing ζ-potential, protein adsorption, and cytotoxicity, necessary attributes for in vivo application of drug-delivery vehicles.
American Psychological Association (APA)
Yogasundaram, Haran& Bahniuk, Markian Stephan& Singh, Harsh-Deep& Aliabadi, Hamidreza Montezari& Uludağ, Hasan& Unsworth, Larry David. 2012. BSA Nanoparticles for siRNA Delivery : Coating Effects on Nanoparticle Properties, Plasma Protein Adsorption, and In Vitro siRNA Delivery. International Journal of Biomaterials،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-482732
Modern Language Association (MLA)
Yogasundaram, Haran…[et al.]. BSA Nanoparticles for siRNA Delivery : Coating Effects on Nanoparticle Properties, Plasma Protein Adsorption, and In Vitro siRNA Delivery. International Journal of Biomaterials No. 2012 (2012), pp.1-10.
https://search.emarefa.net/detail/BIM-482732
American Medical Association (AMA)
Yogasundaram, Haran& Bahniuk, Markian Stephan& Singh, Harsh-Deep& Aliabadi, Hamidreza Montezari& Uludağ, Hasan& Unsworth, Larry David. BSA Nanoparticles for siRNA Delivery : Coating Effects on Nanoparticle Properties, Plasma Protein Adsorption, and In Vitro siRNA Delivery. International Journal of Biomaterials. 2012. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-482732
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-482732