Molecular Analysis of Ciprofloxacin Resistance Mechanisms in Malaysian ESBL-Producing Klebsiella pneumoniae Isolates and Development of Mismatch Amplification Mutation Assays (MAMA) for Rapid Detection of gyrA and parC Mutations
المؤلفون المشاركون
Tay, Sun Tee
Mohd Yusof, Mohd Yasim
al-Marzooq, Farah
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-04-10
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Ninety-three Malaysian extended-spectrum β-lactamase (ESBL)-producing Klebsiella pneumoniae isolates were investigated for ciprofloxacin resistance.
Two mismatch amplification mutation (MAMA) assays were developed and used to facilitate rapid detection of gyrA and parC mutations.
The isolates were also screened for plasmid-mediated quinolone resistance (PMQR) genes including aac(6′)-Ib-cr, qepA, and qnr.
Ciprofloxacin resistance (MICs 4–≥32 μg/mL) was noted in 34 (37%) isolates, of which 33 isolates had multiple mutations either in gyrA alone (n=1) or in both gyrA and parC regions (n=32).
aac(6′)-Ib-cr was the most common PMQR gene detected in this study (n=61), followed by qnrB and qnrS (n=55 and 1, resp.).
Low-level ciprofloxacin resistance (MICs 1-2 μg/mL) was noted in 40 (43%) isolates carrying qnrB accompanied by either aac(6′)-Ib-cr (n=34) or a single gyrA 83 mutation (n=6).
Ciprofloxacin resistance was significantly associated with the presence of multiple mutations in gyrA and parC regions.
While the isolates harbouring gyrA and/or parC alteration were distributed into 11 PFGE clusters, no specific clusters were associated with isolates carrying PMQR genes.
The high prevalence of ciprofloxacin resistance amongst the Malaysian ESBL-producing K.
pneumoniae isolates suggests the need for more effective infection control measures to limit the spread of these resistant organisms in the hospital.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
al-Marzooq, Farah& Mohd Yusof, Mohd Yasim& Tay, Sun Tee. 2014. Molecular Analysis of Ciprofloxacin Resistance Mechanisms in Malaysian ESBL-Producing Klebsiella pneumoniae Isolates and Development of Mismatch Amplification Mutation Assays (MAMA) for Rapid Detection of gyrA and parC Mutations. BioMed Research International،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-484117
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
al-Marzooq, Farah…[et al.]. Molecular Analysis of Ciprofloxacin Resistance Mechanisms in Malaysian ESBL-Producing Klebsiella pneumoniae Isolates and Development of Mismatch Amplification Mutation Assays (MAMA) for Rapid Detection of gyrA and parC Mutations. BioMed Research International No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-484117
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
al-Marzooq, Farah& Mohd Yusof, Mohd Yasim& Tay, Sun Tee. Molecular Analysis of Ciprofloxacin Resistance Mechanisms in Malaysian ESBL-Producing Klebsiella pneumoniae Isolates and Development of Mismatch Amplification Mutation Assays (MAMA) for Rapid Detection of gyrA and parC Mutations. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-484117
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-484117
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر