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Molecular Analysis of Ciprofloxacin Resistance Mechanisms in Malaysian ESBL-Producing Klebsiella pneumoniae Isolates and Development of Mismatch Amplification Mutation Assays (MAMA) for Rapid Detection of gyrA and parC Mutations
Joint Authors
Tay, Sun Tee
Mohd Yusof, Mohd Yasim
al-Marzooq, Farah
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-04-10
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Ninety-three Malaysian extended-spectrum β-lactamase (ESBL)-producing Klebsiella pneumoniae isolates were investigated for ciprofloxacin resistance.
Two mismatch amplification mutation (MAMA) assays were developed and used to facilitate rapid detection of gyrA and parC mutations.
The isolates were also screened for plasmid-mediated quinolone resistance (PMQR) genes including aac(6′)-Ib-cr, qepA, and qnr.
Ciprofloxacin resistance (MICs 4–≥32 μg/mL) was noted in 34 (37%) isolates, of which 33 isolates had multiple mutations either in gyrA alone (n=1) or in both gyrA and parC regions (n=32).
aac(6′)-Ib-cr was the most common PMQR gene detected in this study (n=61), followed by qnrB and qnrS (n=55 and 1, resp.).
Low-level ciprofloxacin resistance (MICs 1-2 μg/mL) was noted in 40 (43%) isolates carrying qnrB accompanied by either aac(6′)-Ib-cr (n=34) or a single gyrA 83 mutation (n=6).
Ciprofloxacin resistance was significantly associated with the presence of multiple mutations in gyrA and parC regions.
While the isolates harbouring gyrA and/or parC alteration were distributed into 11 PFGE clusters, no specific clusters were associated with isolates carrying PMQR genes.
The high prevalence of ciprofloxacin resistance amongst the Malaysian ESBL-producing K.
pneumoniae isolates suggests the need for more effective infection control measures to limit the spread of these resistant organisms in the hospital.
American Psychological Association (APA)
al-Marzooq, Farah& Mohd Yusof, Mohd Yasim& Tay, Sun Tee. 2014. Molecular Analysis of Ciprofloxacin Resistance Mechanisms in Malaysian ESBL-Producing Klebsiella pneumoniae Isolates and Development of Mismatch Amplification Mutation Assays (MAMA) for Rapid Detection of gyrA and parC Mutations. BioMed Research International،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-484117
Modern Language Association (MLA)
al-Marzooq, Farah…[et al.]. Molecular Analysis of Ciprofloxacin Resistance Mechanisms in Malaysian ESBL-Producing Klebsiella pneumoniae Isolates and Development of Mismatch Amplification Mutation Assays (MAMA) for Rapid Detection of gyrA and parC Mutations. BioMed Research International No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-484117
American Medical Association (AMA)
al-Marzooq, Farah& Mohd Yusof, Mohd Yasim& Tay, Sun Tee. Molecular Analysis of Ciprofloxacin Resistance Mechanisms in Malaysian ESBL-Producing Klebsiella pneumoniae Isolates and Development of Mismatch Amplification Mutation Assays (MAMA) for Rapid Detection of gyrA and parC Mutations. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-484117
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-484117