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Phenotypic Definition of the Progenitor Cells with Erythroid Differentiation Potential Present in Human Adult Blood
المؤلفون المشاركون
Migliaccio, Giovanni
Whitsett, Carolyn
Migliaccio, Anna Rita
Masiello, Francesca
Tirelli, Valentina
Ghinassi, Barbara
Sanchez, Massimo
المصدر
العدد
المجلد 2011، العدد 2011 (31 ديسمبر/كانون الأول 2011)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2011-09-26
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
العلوم الطبيعية والحياتية (متداخلة التخصصات)
الملخص EN
In Human Erythroid Massive Amplification (HEMA) cultures, AB mononuclear cells (MNC) generate 1-log more erythroid cells (EBs) than the corresponding CD34pos cells, suggesting that MNC may also contain CD34neg HPC.
To clarify the phenotype of AB HPC which generate EBs in these cultures, flow cytometric profiling for CD34/CD36 expression, followed by isolation and functional characterization (colony-forming-ability in semisolid-media and fold-increase in HEMA) were performed.
Four populations with erythroid differentiation potential were identified: CD34posCD36neg (0.1%); CD34posCD36pos (barely detectable-0.1%); CD34negCD36low (2%) and CD34negCD36neg (75%).
In semisolid-media, CD34posCD36neg cells generated BFU-E and CFU-GM (in a 1 : 1 ratio), CD34negCD36neg cells mostly BFU-E (87%) and CD34posCD36pos and CD34negCD36low cells were not tested due to low numbers.
Under HEMA conditions, CD34posCD36neg, CD34posCD36pos, CD34negCD36low and CD34negCD36neg cells generated EBs with fold-increases of ≈9,000, 100, 60 and 1, respectively, and maturation times (day with >10% CD36highCD235ahigh cells) of 10–7 days.
Pyrenocytes were generated only by CD34neg/CD36neg cells by day 15.
These results confirm that the majority of HPC in AB express CD34 but identify additional CD34neg populations with erythroid differentiation potential which, based on differences in fold-increase and maturation times, may represent a hierarchy of HPC present in AB.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Tirelli, Valentina& Ghinassi, Barbara& Migliaccio, Anna Rita& Whitsett, Carolyn& Masiello, Francesca& Sanchez, Massimo…[et al.]. 2011. Phenotypic Definition of the Progenitor Cells with Erythroid Differentiation Potential Present in Human Adult Blood. Stem Cells International،Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-484189
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Tirelli, Valentina…[et al.]. Phenotypic Definition of the Progenitor Cells with Erythroid Differentiation Potential Present in Human Adult Blood. Stem Cells International No. 2011 (2011), pp.1-9.
https://search.emarefa.net/detail/BIM-484189
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Tirelli, Valentina& Ghinassi, Barbara& Migliaccio, Anna Rita& Whitsett, Carolyn& Masiello, Francesca& Sanchez, Massimo…[et al.]. Phenotypic Definition of the Progenitor Cells with Erythroid Differentiation Potential Present in Human Adult Blood. Stem Cells International. 2011. Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-484189
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-484189
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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