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Phenotypic Definition of the Progenitor Cells with Erythroid Differentiation Potential Present in Human Adult Blood
Joint Authors
Migliaccio, Giovanni
Whitsett, Carolyn
Migliaccio, Anna Rita
Masiello, Francesca
Tirelli, Valentina
Ghinassi, Barbara
Sanchez, Massimo
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-09-26
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Abstract EN
In Human Erythroid Massive Amplification (HEMA) cultures, AB mononuclear cells (MNC) generate 1-log more erythroid cells (EBs) than the corresponding CD34pos cells, suggesting that MNC may also contain CD34neg HPC.
To clarify the phenotype of AB HPC which generate EBs in these cultures, flow cytometric profiling for CD34/CD36 expression, followed by isolation and functional characterization (colony-forming-ability in semisolid-media and fold-increase in HEMA) were performed.
Four populations with erythroid differentiation potential were identified: CD34posCD36neg (0.1%); CD34posCD36pos (barely detectable-0.1%); CD34negCD36low (2%) and CD34negCD36neg (75%).
In semisolid-media, CD34posCD36neg cells generated BFU-E and CFU-GM (in a 1 : 1 ratio), CD34negCD36neg cells mostly BFU-E (87%) and CD34posCD36pos and CD34negCD36low cells were not tested due to low numbers.
Under HEMA conditions, CD34posCD36neg, CD34posCD36pos, CD34negCD36low and CD34negCD36neg cells generated EBs with fold-increases of ≈9,000, 100, 60 and 1, respectively, and maturation times (day with >10% CD36highCD235ahigh cells) of 10–7 days.
Pyrenocytes were generated only by CD34neg/CD36neg cells by day 15.
These results confirm that the majority of HPC in AB express CD34 but identify additional CD34neg populations with erythroid differentiation potential which, based on differences in fold-increase and maturation times, may represent a hierarchy of HPC present in AB.
American Psychological Association (APA)
Tirelli, Valentina& Ghinassi, Barbara& Migliaccio, Anna Rita& Whitsett, Carolyn& Masiello, Francesca& Sanchez, Massimo…[et al.]. 2011. Phenotypic Definition of the Progenitor Cells with Erythroid Differentiation Potential Present in Human Adult Blood. Stem Cells International،Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-484189
Modern Language Association (MLA)
Tirelli, Valentina…[et al.]. Phenotypic Definition of the Progenitor Cells with Erythroid Differentiation Potential Present in Human Adult Blood. Stem Cells International No. 2011 (2011), pp.1-9.
https://search.emarefa.net/detail/BIM-484189
American Medical Association (AMA)
Tirelli, Valentina& Ghinassi, Barbara& Migliaccio, Anna Rita& Whitsett, Carolyn& Masiello, Francesca& Sanchez, Massimo…[et al.]. Phenotypic Definition of the Progenitor Cells with Erythroid Differentiation Potential Present in Human Adult Blood. Stem Cells International. 2011. Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-484189
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-484189