A Fast, Reliable, and Sensitive Method for Detection and Quantification of Listeria monocytogenes and Escherichia coli O157 : H7 in Ready-to-Eat Fresh-Cut Products by MPN-qPCR
المؤلفون المشاركون
Botticella, Giuseppe
Massa, Salvatore
Capozzi, Vittorio
Spano, Giuseppe
Beneduce, Luciano
Russo, Pasquale
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-05-15
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
In the present work we developed a MPN quantitative real-time PCR (MPN-qPCR) method for a fast and reliable detection and quantification of Listeria monocytogenes and Escherichia coli O157:H7 in minimally processed vegetables.
In order to validate the proposed technique, the results were compared with conventional MPN followed by phenotypic and biochemical assays methods.
When L.
monocytogenes and E.
coli O157:H7 were artificially inoculated in fresh-cut vegetables, a concentration as low as 1 CFU g−1 could be detected in 48 hours for both pathogens.
qPCR alone allowed a limit of detection of 101 CFU g−1 after 2 hours of enrichment for L.
monocytogenes and E.
coli O157:H7.
Since minimally processed ready-to-eat vegetables are characterized by very short shelf life, our method can potentially address the consistent reduction of time for microbial analysis, allowing a better management of quality control.
Moreover, the occurrences of both pathogenic bacteria in mixed salad samples and fresh-cut melons were monitored in two production plants from the receipt of the raw materials to the early stages of shelf life.
No sample was found to be contaminated by L.
monocytogenes.
One sample of raw mixed salad was found positive to an H7 enterohemorrhagic serotype.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Russo, Pasquale& Botticella, Giuseppe& Capozzi, Vittorio& Massa, Salvatore& Spano, Giuseppe& Beneduce, Luciano. 2014. A Fast, Reliable, and Sensitive Method for Detection and Quantification of Listeria monocytogenes and Escherichia coli O157 : H7 in Ready-to-Eat Fresh-Cut Products by MPN-qPCR. BioMed Research International،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-484728
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Russo, Pasquale…[et al.]. A Fast, Reliable, and Sensitive Method for Detection and Quantification of Listeria monocytogenes and Escherichia coli O157 : H7 in Ready-to-Eat Fresh-Cut Products by MPN-qPCR. BioMed Research International No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-484728
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Russo, Pasquale& Botticella, Giuseppe& Capozzi, Vittorio& Massa, Salvatore& Spano, Giuseppe& Beneduce, Luciano. A Fast, Reliable, and Sensitive Method for Detection and Quantification of Listeria monocytogenes and Escherichia coli O157 : H7 in Ready-to-Eat Fresh-Cut Products by MPN-qPCR. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-484728
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-484728
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر