A Fast, Reliable, and Sensitive Method for Detection and Quantification of Listeria monocytogenes and Escherichia coli O157 : H7 in Ready-to-Eat Fresh-Cut Products by MPN-qPCR
Joint Authors
Botticella, Giuseppe
Massa, Salvatore
Capozzi, Vittorio
Spano, Giuseppe
Beneduce, Luciano
Russo, Pasquale
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-15
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
In the present work we developed a MPN quantitative real-time PCR (MPN-qPCR) method for a fast and reliable detection and quantification of Listeria monocytogenes and Escherichia coli O157:H7 in minimally processed vegetables.
In order to validate the proposed technique, the results were compared with conventional MPN followed by phenotypic and biochemical assays methods.
When L.
monocytogenes and E.
coli O157:H7 were artificially inoculated in fresh-cut vegetables, a concentration as low as 1 CFU g−1 could be detected in 48 hours for both pathogens.
qPCR alone allowed a limit of detection of 101 CFU g−1 after 2 hours of enrichment for L.
monocytogenes and E.
coli O157:H7.
Since minimally processed ready-to-eat vegetables are characterized by very short shelf life, our method can potentially address the consistent reduction of time for microbial analysis, allowing a better management of quality control.
Moreover, the occurrences of both pathogenic bacteria in mixed salad samples and fresh-cut melons were monitored in two production plants from the receipt of the raw materials to the early stages of shelf life.
No sample was found to be contaminated by L.
monocytogenes.
One sample of raw mixed salad was found positive to an H7 enterohemorrhagic serotype.
American Psychological Association (APA)
Russo, Pasquale& Botticella, Giuseppe& Capozzi, Vittorio& Massa, Salvatore& Spano, Giuseppe& Beneduce, Luciano. 2014. A Fast, Reliable, and Sensitive Method for Detection and Quantification of Listeria monocytogenes and Escherichia coli O157 : H7 in Ready-to-Eat Fresh-Cut Products by MPN-qPCR. BioMed Research International،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-484728
Modern Language Association (MLA)
Russo, Pasquale…[et al.]. A Fast, Reliable, and Sensitive Method for Detection and Quantification of Listeria monocytogenes and Escherichia coli O157 : H7 in Ready-to-Eat Fresh-Cut Products by MPN-qPCR. BioMed Research International No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-484728
American Medical Association (AMA)
Russo, Pasquale& Botticella, Giuseppe& Capozzi, Vittorio& Massa, Salvatore& Spano, Giuseppe& Beneduce, Luciano. A Fast, Reliable, and Sensitive Method for Detection and Quantification of Listeria monocytogenes and Escherichia coli O157 : H7 in Ready-to-Eat Fresh-Cut Products by MPN-qPCR. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-484728
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-484728